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MABF986 Anti-Eosinophil Cationic Protein Antibody, clone 1C8.5/D9

Anti-Eosinophil Cationic Protein Antibody, clone 1C8.5/D9 is an antibody against Eosinophil Cationic Protein for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA, Multiplexing.

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Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: RNASE3, ECP, RNS3 UniProt Number: P12724
MABF986
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), ELISA, MplexMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF986
      DescriptionAnti-Eosinophil Cationic Protein Antibody, clone 1C8.5/D9
      Alternate Names
      • Eosinophil cationic protein
      • ECP
      • Ribonuclease 3
      • RNase 3
      Background InformationEosinophil cationic protein (UniProt P12724; also known as ECP, Ribonuclease 3, RNase 3) is encoded by the RNASE3 (also known as ECP, RNS3) gene (Gene ID 6037) in human. The major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO), are eosinophil effector molecules implicated in host defense, immunoregulatory responses, and in allergic and inflammatory reactions. These highly cationic proteins are stored preformed within the eosinophil secondary granules and released in intact membrane-bound granules during cell lysis or through a complex system of vesiculotubular structures during “piecemeal necrosis”. While eosinophil count and eosinophil surface expression of the activation marker CD69 are significantly correlated with serum concentrations of MBP, EDN and EPO, studies show that they are not correlated with ECP level. On the other hand, eosinophilic disease patients with normal eosinophil count show significantly increased concentrations of MBP, ECP, EDN, and EPO compared to normal donors. The measurement of MBP, ECP, EDN, and EPO levels thus provides a better predictor than eosinophil count alone in disease diagnosis. ECP is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature (a.a. 28-160) protein.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Eosinophil Cationic Protein Antibody, clone 1C8.5/D9 is an antibody against Eosinophil Cationic Protein for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA, Multiplexing.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • ELISA
      • Multiplexing
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected eosinophil cationic protein in 50 µg of human liver and lung tissue lysates.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Eosinophil Cationic Protein in human small intestine and human colon tissue.
      ELISA Analysis: A representative lot was employed as the capture antibody for the detection of purified human ECP, as well as ECP in serum samples from individuals with eosinophilic disorders by sandwich ELISA (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),
      Multiplexing Analysis: A representative lot was employed as the capture antibody in a bead-based multiplex assay for the detection of purified human ECP as well as ECP in serum samples from healthy donors and individuals with eosinophilic disorders. Prior reduction and alkylation of the serum samples (by DTT and iodoacetamide, respectively) resulted in higher EDN levels (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),
      Biological Information
      ImmunogenPurified human ECP.
      Clone1C8.5/D8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1C8.5/D9 specifically reacted with ECP, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNASE3
      • ECP
      • RNS3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~20 kDa observed. Target band appears larger than the calculated molecular weights of 18.39 kDa (pro-form) and 15.52 kDa (mature) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected eosinophil cationic protein in 50 µg of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF986 04055977334333