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GR01L
Sigma-AldrichAnti-EGFR (Ab-1) Mouse mAb (528)
Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
More>>Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies. Less<<
Anti-EGFR (Ab-1) Mouse mAb (528): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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Description
Overview
Recognizes the EGF receptor protein in A431 cells and normal skin.
Catalogue Number
GR01L
Brand Family
Calbiochem®
Synonyms
Anti-Epidermal Growth Factor Receptor
References
References
Hunter T. 1984. Nature311, 414. Masui, H., et al. 1984. Cancer Res.44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Product Information
Form
Lyophilized
Formulation
Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
Flow Cytometry (5 µg/ml) Frozen Sections (2.5 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (see comments and application references) Paraffin Sections (see application references)
Application Comments
This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
partially purified, human EGFR from human A431 cells
Immunogen
Human
Clone
528
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia
GTIN
GR01L-100UG
04055977220155
Documentation
Anti-EGFR (Ab-1) Mouse mAb (528) Ficha datos de seguridad (MSDS)
Anti-EGFR (Ab-1) Mouse mAb (528) Certificados de análisis
Cargo
Número de lote
GR01L
Referencias bibliográficas
Visión general referencias
Hunter T. 1984. Nature311, 414. Masui, H., et al. 1984. Cancer Res.44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Ficha técnica
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
25-October-2007 JSW
Synonyms
Anti-Epidermal Growth Factor Receptor
Application
Flow Cytometry (5 µg/ml) Frozen Sections (2.5 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (see comments and application references) Paraffin Sections (see application references)
Description
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
Background
Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
Host
Mouse
Immunogen species
Human
Immunogen
partially purified, human EGFR from human A431 cells
Clone
528
Isotype
IgG2a
Species
human, not mouse, not rat
Positive control
A-431 cells or skin tissue
Form
Lyophilized
Formulation
Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
Preservative
None
Comments
This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
Toxicity
Standard Handling
References
Hunter T. 1984. Nature311, 414. Masui, H., et al. 1984. Cancer Res.44, 1002. Reynolds, F. H., Jr., et al. 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Application references
Original Clone
Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA80, 1337.
Neutralization Studies
Gill, G., et al. 1984. J. Biol. Chem.259, 7761.
Kawamoto, T., et al. 1984. J. Biol Chem.259, 7761.
Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA80, 1337.
Sato, J.D., et al. 1983. Molec. Biol. Med.1, 511.
Paraffin Sections
Burton B.C., et al. 2002. Clin. Cancer Res.1, 180.
Mishima, K., et al. 2001. Cancer Res.61, 7703.
Pilcher B.K., et al. 1999. J. Biol. Chem.274, 10372.
