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NA49 Anti-E2F1 (Ab-1) Mouse mAb (KH95)

Anti-E2F1 (Ab-1) Mouse mAb (KH95): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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      Descripción

      Replacement Information

      Tabla espec. clave

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the E2F1 protein is found in most cell types.
      Catalogue NumberNA49
      Brand Family Calbiochem®
      References
      ReferencesCampanero, M.R. and Flemington, E.K. 1997. Proc. Natl. Acad. Sci. USA 94, 2221.
      DeGregori, J., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 7245.
      Fan, J. and Bertino, J.R. 1997. Oncogene 14, 1191.
      Khleif, S.N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 43501.
      Neuman, E., et al. 1996. Gene 173, 163.
      Slansky, J.E. and Farnham, P.J. 1996. Curr. Top. Microbiol. Immunol. 208, 1.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Positive controlHeLa or Y79 cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Not Immunofluorescence
      Not Immunoprecipitation
      Application NotesImmunoblotting (2.5 µg/ml)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Application CommentsThe E2F1 protein is found in most cell types. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human E2F1
      ImmunogenHuman
      CloneKH95
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      NA49 0

      Documentation

      Anti-E2F1 (Ab-1) Mouse mAb (KH95) Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-E2F1 (Ab-1) Mouse mAb (KH95) Certificados de análisis

      CargoNúmero de lote
      NA49

      Referencias bibliográficas

      Visión general referencias
      Campanero, M.R. and Flemington, E.K. 1997. Proc. Natl. Acad. Sci. USA 94, 2221.
      DeGregori, J., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 7245.
      Fan, J. and Bertino, J.R. 1997. Oncogene 14, 1191.
      Khleif, S.N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 43501.
      Neuman, E., et al. 1996. Gene 173, 163.
      Slansky, J.E. and Farnham, P.J. 1996. Curr. Top. Microbiol. Immunol. 208, 1.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationImmunoblotting (2.5 µg/ml)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      DescriptionPurified mouse monoclonal antibody antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the E2F1 protein.
      BackgroundE2F transcription activity is the result of regulation by a family of heterodimers that are encoded by distinct genes. The different members of the E2F family form complexes with the DP1 protein and it is this complex which is responsible for the regulatory activity. The gene for E2F1 was the first of the E2F family to be cloned and was shown to map to chromosome 20q11. The gene contains 7 exons and is regulated at the mRNA levels through changes in transcription. E2F1 has been shown to regulate both G1 and S phase cyclins and in particular there is evidence for a type of feedback regulatory loop between E2F and cyclin D1. Overexpression of E2F1 has been shown to lead to the induction of apoptosis possibly through the inhibition of cyclinD1 dependent kinase activity coupled with the induction of a p16 related transcript. In addition to the transcription level regulation of E2F1, there is also control of E2F1 protein levels by the ubiquitin-proteosome dependent degradation pathway. Ubiquitination is blocked by Rb stabilizing the E2F1 complex formation and directly controlling cell cycle progression.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human E2F1
      CloneKH95
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa or Y79 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsThe E2F1 protein is found in most cell types. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCampanero, M.R. and Flemington, E.K. 1997. Proc. Natl. Acad. Sci. USA 94, 2221.
      DeGregori, J., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 7245.
      Fan, J. and Bertino, J.R. 1997. Oncogene 14, 1191.
      Khleif, S.N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 43501.
      Neuman, E., et al. 1996. Gene 173, 163.
      Slansky, J.E. and Farnham, P.J. 1996. Curr. Top. Microbiol. Immunol. 208, 1.