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MABF2774-25UG Anti-Dengue Virus 2 NS1 Antibody, clone 164

This mouse monoclonal Anti-Dengue Virus 2 NS1, clone 164, Cat. No. MABF2774, is a mouse monoclonal antibody that detects Dengue virus NS1 protein and is tested for use in ELISA.

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MABF2774-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABF2774-25UG
      DescriptionAnti-Dengue Virus 2 NS1 Antibody, clone 164
      Alternate Names
      • Genome polyprotein
      • NS1 protein
      • DENV2 NS1
      Background InformationGenome polyprotein (UniProt: P29990) following its synthesis is cleaved into 13 different proteins in Dengue virus 2. Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. (Ref.: Chen, RU et al. (2018). J. Biomed. Sci. 25(1); 58; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-Dengue Virus 2 NS1, clone 164, Cat. No. MABF2774, is a mouse monoclonal antibody that detects Dengue virus NS1 protein and is tested for use in ELISA.
      Key Applications
      • ELISA
      Application NotesELISA Analysis: A representative lot detected Dengue Virus 2 NS1 in ELISA applications (Bosch, I., et. al. (2020). PLoS Negl Trop Dis. 14(6):e0008203).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant Dengue Virus 2 NS1 protein.
      EpitopeN-terminal half
      Clone164
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 164 is a mouse monoclonal antibody that detects NS1 protein of Dengue virus 2. It targets an epitope within the N-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteDengue Virus 2
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~48 kDa calculated for NS1 protein and 379.547 kDa for the complete genome polyprotein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with DENV2 NS1 recombinant protein.

      ELISA Analysis: 1.0 µg/mL of this antibody detected recombinant Dengue Virus 2 NS1 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF2774-25UG 04065266020670