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MABF1980-100UL Anti-Complement receptor C5aR (CD88) Antibody, clone S5/1

Anti-Complementreceptor C5aR (CD88), clone S5/1, Cat. No. MABF1980 is a mouse monoclonal antibody that detects C5a anaphylatoxin chemotactic receptor 1 and has been tested for use in ELISA, Flow Cytometry, Immunoprecipitation, Inhibition/Activity assays, andn Western Blotting.

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MABF1980-100UL
100 µL  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABF1980-100UL
      DescriptionAnti-Complement receptor C5aR (CD88) Antibody, clone S5/1
      Alternate Names
      • C5a anaphylatoxin chemotactic receptor 1
      • C5a anaphylatoxin chemotactic receptor
      • C5a-R
      • CD88
      Background InformationC5a anaphylatoxin chemotactic receptor 1 (UniProt: P21730; also known as C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88) is encoded by the C5AR1 (also known as C5AR, C5R1) gene (Gene ID: 728) in human. C5aR is a multi-pass membrane protein that serves as a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. It exists as a homodimer, but can also form higher-order oligomers. C5a interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region, which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, and intracellular calcium release and superoxide anion production. Chemotaxis inhibitory protein of Staphylococcus aureus (CHIPS), a 121-residue protein, is reported to bind to the N-terminus of the C5aR (aa 1-35) with nanomolar affinity and inhibits C5a-mediated responses in human leukocytes. Sulfation at Tyrosine 14 is shown to be important for CHIPS binding. Upon C5a binding, C5aR) undergoes rapid phosphorylation on the six serine residues present in the C-terminal region followed by desensitization and internalization. The key residues involved in this process are Serine 334 and Serine 338. Phosphorylated C5aR is shown to co-localize with beta-arrestin 1 and 2 in cytoplasmic vesicles. Although C5aR activation is able to promote a loose association with beta-arrestins, but phosphorylation of either Serine 334)/338 or Serine 327)/338 is reported to be essential for the formation of a persistent complex. (Ref.: Ippel, JH., et al., (2009). J. Biol. Chem. 284(18); 12363 12372; Braun, L., et al. (2003). J. Biol. Chem. 278(6); 4277-4285).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Applications
      ApplicationAnti-Complementreceptor C5aR (CD88), clone S5/1, Cat. No. MABF1980 is a mouse monoclonal antibody that detects C5a anaphylatoxin chemotactic receptor 1 and has been tested for use in ELISA, Flow Cytometry, Immunoprecipitation, Inhibition/Activity assays, andn Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Inhibits Activity/Function
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected Complementreceptor C5aR (CD88) in ELISA applications (Pollok-Kopp, B., et. al. (2007). J Biol Chem. 282(7):4345-53).

      Inhibits Activity/Function Analysis: A representative lot detected Complementreceptor C5aR (CD88) in Inhibits Activity applications (Oppermann, M., et. al. (1993). J Immunol. 151(7):3785-94).

      Flow Cytometry Analysis: A representative lot detected Complementreceptor C5aR (CD88) in Flow Cytometry applications (Oppermann, M., et. al. (1993). J Immunol. 151(7):3785-94; Pollok-Kopp, B., et. al. (2007). J Biol Chem. 282(7):4345-53).

      Immunoprecipitation Analysis: A representative lot detected Complementreceptor C5aR (CD88) in Immunoprecipitation applications (Pollok-Kopp, B., et. al. (2007). J Biol Chem. 282(7):4345-53).
      Biological Information
      ImmunogenBSA-conjugated linear peptide corresponding to the first 31 amino acids from the N-terminal region of human C5a anaphylatoxin chemotactic receptor 1.
      EpitopeN-terminus
      CloneS5/1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone S5/1 specifically detects C5a anaphylatoxin chemotactic receptor 1 (C5aR). It targets an epitope within the first 31 amino acids from the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • C5AR1
      • C5AR
      • C5R1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~100-110 kDa observed; 39.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in two BSA-conjugated C5aR peptides (aa 1-31 and 327-344) phosphorylated at Serine 332, 334, and 338.

      Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Complementreceptor C5aR (CD88) in two BSA-conjugated C5aR peptides (aa 1-31 and 327-344) phosphorylated at Serine 332, 334, and 338
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF1980-100UL 04054839468995

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