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AB5873-I Anti-Catechol O-methyltransferase/COMT Antibody

This Anti-Catechol O-methyltransferase Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of COMT.

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Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: COMT UniProt Number: P21964
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AB5873-I
100 μg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5873-I
      ReplacesAB5873
      DescriptionAnti-Catechol O-methyltransferase/COMT Antibody
      Alternate Names
      • Catechol O-methyltransferase
      Background InformationCatechol O-methyltransferase (EC 2.1.1.6; UniProt P21964) is encoded by the COMT gene (Gene ID 1312) in human. Catechol-O-methyltransferase initiates the metabolic breakdown of catecholamines (including the neurotransmitters dopamine, epinephrine, and norepinephrine) by catalyzing the transfer of a methyl group from S-adenosylmethionine (SAM) to catecholamines in the presence Mg++. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to endogenous substances, COMT is also important in the metabolism of catechol drugs. Two isoforms exist as a result of alternative initiation codon usage. The membrane-bound isoform or MB-COMT contains a short N-terminal cytoplasmic tail (a.a. 1-6), a transmembrane segment (a.a. 7-26), and a large C-terminal extracellular region (a.a. 27-271), while the soluble or S-COMT isoform lacks the N-terminal 50-amino acid sequence of MB-COMT. MB-COMT and S-COMT differ in their substrate affinity and capacities. Despite having a lower capacity than S-COMT, MB-COMT has around a 10-fold greater affinity for dopamine and noradrenaline, and therefore is likely to be better suited for metabolizing catecholamines at the concentrations found in brain. The influence of the Val(158)Met polymorphic form of catechol-o-methyltransferase (COMT) on brain activation and functional connectivity is the subject of numerous studies.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Catechol O-methyltransferase Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of COMT.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Catechol O-methyltransferase/COMT in human prostate cancer, normal liver and kidney tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an extracellular domain sequence of human catechol O-methyltransferase.
      EpitopeExtracellular domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody was raised against a sequence present in both the membrane-bound (MB-COMT) and the soluble (S-COMT) spliced isoforms of human catechol O-methyltransferase reported by UniProt (P21964).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Horse, Porcine, Rhesus Macaque, Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COMT
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~28 kDa observed. 30.04 kDa (human MB-COMT) and 24.45 kDa (human S-COMT) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human fetal liver tissue lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected Catechol O-methyltransferase/COMT in 10 µg of human fetal liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      AB5873-I 04055977351453