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MABF1011 Anti-CMKLR1 Antibody, clone BZ194

MABF1011
100 μg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF1011
      DescriptionAnti-CMKLR1 Antibody, clone BZ194
      Alternate Names
      • Chemokine-like receptor 1
      • Cmklr1
      • G-protein coupled receptor 27
      • G-protein coupled receptor DEZ
      Background InformationChemokine-like receptor 1 (UniProt P97468; also known as G-protein coupled receptor 27, G-protein coupled receptor DEZ) is encoded by the Cmklr1 (also known as Dez, Gpcr27, mcmklr1) gene (Gene ID 14747) in murine species. Cmklr1 is a 7-transmembrane G-protein-coupled receptor (GPCR) that is activated by chemoattractant chemerin. Chemerin is shown to induce the migration of macrophages and dendritic cells (DCs) in vitro, while in vivo studies using Cmklr1-knockout mice indicate an anti-inflammatory role of this receptor, possibly due to the recruitment of tolerogenic plasmacytoid DCs. In addtion, chemerin/Cmklr1 interaction is also reported to promote adipogenesis and angiogenesis. The anti-inflammatory lipid mediator, resolving E1, is another known Cmklr1 ligand that functions to prevent leukocyte infiltration and proinflammatory gene expression.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CMKLR1 Antibody, clone BZ194 is an antibody against CMKLR1 for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A representative lot detected Cmklr1 expression on CD11c+/CD11b+/B220- myeloid dendritic cells (mDC) from the spinal cords of mice with acute experimental autoimmune encephalomyelitis (EAE) (Graham, K.L., et al. (2009). J. Immunol.183(10):6717–6723).
      Flow Cytometry Analysis: A representative lot detected exogenously expressed mouse Cmklr1, but not human Cmklr1, on the respective murine pre-B lymphoma L1.2 transfectants (Zabel, B.A., et al. (2006). Exp. Hematol. 34(8):1106-1114).
      Flow Cytometry Analysis: A representative lot detected Cmklr1 immunoreactivity among a subpopulation of CD11c+ mouse bone marrow cells, while no Cmklr1 immunoreactivity was detected among TCR-β-/CD19-/CD11c+ murine dendrditic cells (DCs) from bone marrow, spleen, lymph nodes, and blood (Zabel, B.A., et al. (2006). Exp. Hematol. 34(8):1106-1114).
      Flow Cytometry Analysis: A representative lot detected Cmklr1 immunoreactivity associated with murine F4/80+/CD11+ macrophages from peritoneal cavity, pleural cavity, and peripheral blood (Zabel, B.A., et al. (2006). Exp. Hematol. 34(8):1106-1114).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminal extracellular region of mouse CMKLR1.
      EpitopeN-terminal extracellular region
      CloneBZ194
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone BZ194 specifically stains transfected L1.2 murine pre-B lymphoma cells that express mouse, but not human Cmklr1. Clone BZ194 does not stain murine cells known to express CXCR1 through 6 or CCR1 through 10 (Zabel, B.A., et al. (2006). Exp. Hematol. 34(8):1106-1114).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cmklr1
      • Dez
      • Gpcr27
      • mcmklr1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight41.8 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse peritoneal cells.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected CMKLR1 on mouse peritoneal cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF1011 04055977297454

      Documentation

      Anti-CMKLR1 Antibody, clone BZ194 Certificados de análisis

      CargoNúmero de lote
      Anti-CMKLR1, clone BZ194 -Q2618560 Q2618560

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Chemokine-like receptor-1 expression by central nervous system-infiltrating leukocytes and involvement in a model of autoimmune demyelinating disease.
      Graham, KL; Zabel, BA; Loghavi, S; Zuniga, LA; Ho, PP; Sobel, RA; Butcher, EC
      Journal of immunology (Baltimore, Md. : 1950)  183  6717-23  2009

      Mostrar resumen
      19864606 19864606
      Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands.
      Zabel, BA; Ohyama, T; Zuniga, L; Kim, JY; Johnston, B; Allen, SJ; Guido, DG; Handel, TM; Butcher, EC
      Experimental hematology  34  1106-14  2005

      Mostrar resumen
      16863918 16863918

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