Millipore Sigma Vibrant Logo

MABN2265 Anti-CHD-8 Antibody, clone 7F4.1

Anti-CHD-8, clone 7F4.1, Cat. No. MABN2265, is a highly specific mouse monoclonal antibody that targets Chromodomain-helicase-DNA-binding protein 8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CHD-8 Antibody, clone 7F4.1: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Gene Symbol: CHD8, HELSNF1, KIAA1564 UniProt Number: Q9HCK8
View Products on Sigmaaldrich.com
MABN2265
100 μg  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2265
      DescriptionAnti-CHD-8 Antibody, clone 7F4.1
      Alternate Names
      • Chromodomain-helicase-DNA-binding protein 8
      • EC: 3.6.4.12
      • ATP-dependent helicase CHD8
      • Helicase with SNF2 domain 1
      Background InformationChromodomain-helicase-DNA-binding protein 8 (UniProt: Q9HCK8; also known as EC: 3.6.4.12, CHD-8, ATP-dependent helicase CHD8, Helicase with SNF2 domain 1) is encoded by the CHD8 (also known as HELSNF1, KIAA1564) gene (Gene ID: 57680) in human. CHD-8 is a member of the SNF2/RAD54 helicase family. Two isoforms of CHD-8 have been reported that are produced by alternative splicing. It is a DNA helicase that acts as a chromatin remodeling factor and regulates transcription. It also acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. CHD-8 is reported to suppress p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity and also acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin activity. It negatively regulates beta-catenin-targeted gene expression by being recruited specifically to the promoter regions of several beta-catenin responsive genes. CHD-8 is involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Mutations in CHD8 gene are associated with Autism 18 that is characterized by impairments in reciprocal social interaction and communication and developmental abnormalities.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CHD-8, clone 7F4.1, Cat. No. MABN2265, is a highly specific mouse monoclonal antibody that targets Chromodomain-helicase-DNA-binding protein 8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-8 in human cervical cancer and human tonsil tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 112 amino acids rom the C-terminal half of human Chromodomain-helicase-DNA-binding protein 8. The immunogen sequence is conserved in both isoforms.
      Clone7F4.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7F4.1 detects human Chromodomain-helicase-DNA-binding protein 8 (CHD8). It targets an epitope within 112 amino acids from the C-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHD8
      • HELSNF1
      • KIAA1564
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight270 and 300 kDa observed; 290.52 and 262.35 kDa calculated for isoforms 1 and 2, respectively. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-8 in 10 µg of SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABN2265 04054839412400