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MABT1525-100UG Anti-CDH17 Antibody, clone Lic5

Anti-CDH17, clone Lic5, Cat. No. MABT1525, is a mouse monoclonal antibody that detects Cadherin-17 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Inhibition Assays, and Western Blotting.

MABT1525-100UG
100 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABT1525-100UG
      DescriptionAnti-CDH17 Antibody, clone Lic5
      Alternate Names
      • Intestinal peptide-associated transporter HPT-1
      • Liver-intestine cadherin
      • LI-cadherin
      Background InformationCadherin-17 (UniProt: Q12864; also known as Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, LI-cadherin) is encoded by the CDH17 gene (Gene ID: 1015) in human. Cadherin-17 is a single-pass type I membrane protein that is expressed in the gastrointestinal tract and pancreatic duct. It belongs to the non-classical cadherin with distinct structural feature of having seven cadherin repeats at its extracellular amino-terminus. Three calcium ions are usually bound at the interface of each cadherin repeat and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Cadherin-17 is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature form that contains a long extracellular domain (aa 23-787), a transmembrane domain (aa 788-808), and a cytoplasmic domain (aa 809-832). It differs from classical cadherins in having a short cytoplasmic tail at its C-terminus. Cadherin-17 is reported to be a oncofetal protein that is associated with poor prognostic outcome in subjects with hepatocellular carcinoma. Its levels can be up-regulated or down-regulated in different cancers, including pancreatic, gastric and colorectal cancers. Clone Lic5 is shown to reduce the levels of beta-catenin and in hepatocellular carcinoma cells and administration of Lic5 is reported to induce the activation of caspase-8 and 9 in these cells. In addition, it is also shown to suppress tumor growth of MHCC97L cells in murine models. (Ref.: Wang, Y., et al. (2013). PLoS One. 8(8); e72386).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2bk in buffer containing PBS without azide.
      Applications
      ApplicationAnti-CDH17, clone Lic5, Cat. No. MABT1525, is a mouse monoclonal antibody that detects Cadherin-17 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Inhibition Assays, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Inhibition
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected CDH17 in Immunohistochemistry applications (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).

      Western Blotting Analysis: A representative lot detected CDH17 in Western Blotting applications (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).

      Immunocytochemistry Analysis: A representative lot detected CDH17 in Immunocytochemistry applications (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).

      Inhibition Analysis: A representative lot reduced CDH17 expression in a dose-dependent manner and suppressed -catenin signaling. (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).
      Biological Information
      ImmunogenRecombinant fragment corresponding to extracellular domains 1 and 2 of human Cadherin-17 (CDH17).
      Epitopeextracellular domain
      CloneLic5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone Lic5 is a mouse monoclonal antibody that specifically detects human Cadherin-17. It targets an epitope within the extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH17
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight92.22 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human small intestine tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Cadherin-17 (CDH17) in glandular cells of human small intestine tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABT1525-100UG 04061841068655

      Documentation

      Anti-CDH17 Antibody, clone Lic5 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-CDH17 Antibody, clone Lic5 Certificados de análisis

      CargoNúmero de lote
      Anti-CDH17, clone Lic5 - Q3286539 Q3286539

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Anti-cadherin-17 antibody modulates beta-catenin signaling and tumorigenicity of hepatocellular carcinoma.
      Wang, Y; Shek, FH; Wong, KF; Liu, LX; Zhang, XQ; Yuan, Y; Khin, E; Hu, MY; Wang, JH; Poon, RT; Hong, W; Lee, NP; Luk, JM
      PLoS One  8  e72386  2013

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