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MABF3056-100UG Anti-CD36 Antibody, clone ZND36-6

Anti-CD36, clone ZND36-6, Cat. No. MABF3056, is a rat monoclonal antibody that detects CD36 and is tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition Assay.

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MABF3056-100UG
100 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABF3056-100UG
      DescriptionAnti-CD36 Antibody, clone ZND36-6
      Alternate Names
      • Platelet glycoprotein 4
      • Glycoprotein IIIb
      • GPIIIB
      • PAS IV
      • PAS-4
      • Platelet glycoprotein IV
      • GPIV
      • SR-B2
      Background InformationPlatelet glycoprotein 4 (UniProt: Q08857; also known as Glycoprotein IIIb, GPIIIB, PAS IV, PAS-4, Platelet glycoprotein IV, GPIV, SR-B2 CD36) is encoded by the cd36 gene (Gene ID: 12491) in murine species. CD36 us a multi-pass membrane glycoprotein with two cytoplasmic domains (aa 1-7 and 462-472), two transmembrane domains (aa 8-29 and 440-461), and one extracellular domain (aa 30-439). It is highly expressed in intestine on the luminal surface of enterocytes. Its expression is also observed in the apical side of lingual taste bunds of circumvallate papillae. Its expression is also reported on monocyte-derived inflammatory macrophages that infiltrate into alveolar spaces, but on alveolar resident macrophages. It serves as a multifunctional protein and acts as a receptor for a broad range of ligands, such as thrombospondin, fibronectin, collagen, oxidized low-density lipoprotein, and bacterial diacylated lipopeptides. It is generally multivalent and can engage multiple receptors simultaneously, resulting in the formation of CD36 clusters that initiate signal transduction and internalization of receptor-ligand complexes. Cellular responses to its ligands are reported to be involved in angiogenesis, inflammatory response, fatty acid metabolism, and dietary fat processing in the intestine. CD36 is shown to bind long-chain fatty acids and facilitate their transport into cells, thereby promoting muscle lipid utilization, gut fat absorption, and adipose energy storage. It is also an important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis. CD36 also reported to be a sensor for lipoteichoic acid, a Gram-positive cell wall component, and transmits inflammatory signals in conjunction with TLR2/TLR6 heterodimer and this cluster triggers signaling leading to NF-kB-dependent production of TNF-a via MYD88 signaling pathway. Clone ZND36-6 is an anti-SR-B2 (CD36) blocking antibody that has been used in pulmonary inflammation induced by exposure to silica. (Ref.: Tsugita, M., et al. (2017). Cell Rep. 18(5); 1298-1311; Stewart, CR., et al, (2010). Nat. Immunol. 11(2):155-161; Endemann, G., et al (1993). J. Biol. Chem. 268(16):11811-11816).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CD36, clone ZND36-6, Cat. No. MABF3056, is a rat monoclonal antibody that detects CD36 and is tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition Assay.
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      • Inhibition
      Application NotesTested Applications

      Flow Cytometry: A representative lot detected CD36 in Flow Cytometry applications (Tsugita, M., et al. (2017). Cell Rep. 18(5):1298-1311; Omori, S., et al. (2021). Cell Rep. 34(6):108734).

      Inhibition Assay: A representative lot of this antibody partially inhibited IL-1b secretion by bone marrow derived macrophages. (Tsugita, M., et al. (2017). Cell Rep. 18(5):1298-1311).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenNIH3T3 cells expressing murine CD36 (SR-B2).
      EpitopeExtracellular domain
      CloneZND36-6
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone ZND36-6 is a rat monoclonal antibody that detects murine CD36 (SR-B2).
      IsotypeIgG2a
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cd36
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight52.70 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in NIH 3T3 cells.

      Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected CD36 in NIH 3T3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF3056-100UG 04065267463834