Millipore Sigma Vibrant Logo

MABN1785 Anti-CD166 (ALCAM) Antibody, clone TAG-1A3

Anti-CD166 (ALCAM) Antibody, clone TAG-1A3, Cat. No. MABN1785, is a highly specific mouse monoclonal antibody that targets CD166 and has been tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

View Products on Sigmaaldrich.com
MABN1785
200 µL  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information
      Description
      Catalogue NumberMABN1785
      DescriptionAnti-CD166 (ALCAM) Antibody, clone TAG-1A3
      Alternate Names
      • Activated leukocyte cell adhesion molecule
      • CD166 antigen
      Background InformationCD166 antigen (UniProt: Q13740; also known as Activated leukocyte cell adhesion molecule, CD166) is encoded by the ALCAM (also known as MEMD) gene (Gene ID: 214) in human. CD166 is a cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6 and homotypic cell-cell contacts. It also promotes T-cell activation and proliferation via its interactions with CD6 and is shown to be involved in the formation and maturation of the immunological synapse via its interactions with CD6. CD166 mediates attachment of dendritic cells onto endothelial cells via homotypic interaction and inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. It has an extracellular (aa 28-527), transmembrane (aa 528-549), and a cytoplasmic (aa 550-583) domain. CD166 is expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. It is also detected on hematopoietic stem cells derived from umbilical cord blood, on lymph vessel endothelial cells, skin and tonsil, and on peripheral blood monocytes. Lower levels of CD166 are detected in spleen, placenta, and liver. CD166 is also detected in highly metastasizing melanoma cell lines.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CD166 (ALCAM) Antibody, clone TAG-1A3, Cat. No. MABN1785, is a highly specific mouse monoclonal antibody that targets CD166 and has been tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Flow Cytometry
      • Immunocytochemistry
      • Western Blotting
      • Immunofluorescence
      Application NotesFlow Cytometry Analysis: A representative lot detected CD166 (ALCAM) in Flow Cytometry applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

      Immunocytochemistry Analysis: A representative lot detected CD166 (ALCAM) in Immunocytochmistry applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

      Immunoprecipitation Analysis: A representative lot detected CD166 (ALCAM) in Immunoprecipitation applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

      Flow Cytometry Analysis: A represetative lot detected CD166 (ALCAM) in human embryonic stem cells, human corneal stromal fibroblast (hCSF) cells, immortalized human corneal endothelial cells (B4G12), and human endothelial cells from donors (2) (Courtesy of Dr. Vanessa Ding, Ph.D. and Dr. Andre Choo, Ph.D, BTI, Singapore).

      Immunofluorescence Analysis: A representative lot detected CD166 (ALCAM) in Immunofluorescence applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

      Western Blotting Analysis: A representative lot detected CD166 (ALCAM) in Western Blotting applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).
      Biological Information
      ImmunogenCorneal endothelial cells (hCENC) isolated from donated human cadaves and immunized with monophosphoryl-lipid A trehalose dicorynomycolate (MPLCTDM) adjuvant.
      CloneTAG-1A3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone TAG-1A3 specifically detects CD166 in human.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ALCAM
      • MEMD
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~80 kDa observed; 65.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIdentity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be human IgG1 .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABN1785 04054839232602

      Productos y aplicaciones relacionados