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MAB3224 Anti-Basal Cell Cytokeratin Antibody

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MAB3224
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, Ch, H, Ht, Po, R, Rb, Qu, GpFC, ICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3224
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Basal Cell Cytokeratin Antibody
      Alternate Names
      • Cytokeratin 5 and others
      Background InformationCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Purified immunoglobulin in buffer with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Basal Cell Cytokeratin Antibody detects level of Basal Cell Cytokeratin & has been published & validated for use in FC, IC, IH & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:100-1:1,000
      Immunohistochemistry on frozen tissue sections: 1:100-1:200
      Immunocytochemistry: 1:100-1:200
      Flow cytometry: 1:100-1:200
      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman cytokeratins.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB3224 is a broadly reacting cytokeratin antibody which reacts with cytokeratin 5 among others. It stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cell in the prostate epithelium.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Chicken
      • Human
      • Hamster
      • Pig
      • Rat
      • Rabbit
      • Quail
      • Guinea Pig
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
      Gene Symbol
      • KRT5
      • DDD
      • CK5
      • K5
      • EBS2
      • Keratin-5
      • KRT5A
      • Cytokeratin-5
      • CK-5
      UniProt Number
      UniProt SummarySIZE: 590 amino acids; 62378 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
      DISEASE: SwissProt: P13647 # Defects in KRT5 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 131960]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%) and it is characterized by blistering within the basal cell layer. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal-epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema [MIM:609352]. EBS with migratory circinate erythema is milder than the DM-EBS phenotype but involves an unusual migratory circinate erythema with multiple vesicles on the area affected by the erythema. The lesions, which appeared from birth primarily on the hands, feet, and legs but spared the nails, ocular epithelia, and mucosae, healed with brown pigmentation but no scarring. Electron microscopy findings were distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is characterized by additional reticular hyperpigmentation. & Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
      SIMILARITY: SwissProt: P13647 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MAB3224 04053252502903

      Documentation

      Anti-Basal Cell Cytokeratin Antibody Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Basal Cell Cytokeratin Antibody Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-CYTOKERATIN, BASAL CELL MONOCLONAL ANTIBODY - 2119720 2119720
      MOUSE ANTI-CYTOKERATIN, BASAL CELL MONOCLONAL ANTIBODY - 2381766 2381766
      MOUSE ANTI-CYTOKERATIN, BASAL CELL MONOCLONAL ANTIBODY - 2392315 2392315
      MOUSE ANTI-CYTOKERATIN, BASAL CELL - 3286831 3286831
      MOUSE ANTI-CYTOKERATIN, BASAL CELL - 3352024 3352024
      MOUSE ANTI-CYTOKERATIN, BASAL CELL - 3506474 3506474
      MOUSE ANTI-CYTOKERATIN, BASAL CELL - 3874843 3874843
      MOUSE ANTI-CYTOKERATIN, BASAL CELL - 4202314 4202314
      MOUSE ANTI-CYTOKERATIN, BASAL CELL -2529360 2529360
      MOUSE ANTI-CYTOKERATIN, BASAL CELL -2610537 2610537

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Oct4A is expressed by a subpopulation of prostate neuroendocrine cells.
      Sotomayor, P; Godoy, A; Smith, GJ; Huss, WJ
      The Prostate  69  401-10  2009

      Mostrar resumen Artículo Texto completo
      19058139 19058139
      Simultaneous cell death and desquamation of the embryonic diffusion barrier during epidermal development.
      Manuela Saathoff, Barbara Blum, Thomas Quast, Gregor Kirfel, Volker Herzog
      Experimental cell research  299  415-26  2004

      Mostrar resumen
      15350540 15350540
      Development of a novel monoclonal antibody recognizing basal cells of human squamous epithelia.
      Jiro Hitomi, Fumio Ishizaki, Eiji Kimura, Nobuyuki Sato
      Archives of histology and cytology  65  201-8  2002

      Mostrar resumen
      12164343 12164343

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies