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MABN679 Anti-BHMT Antibody, clone 8C11H5

Anti-BHMT Antibody, clone 8C11H5 is a highly specific mouse monoclonal antibody, that targets Betaine--homocysteine S-methyltransferase 1 & has been tested in IHC & western blotting.

Anti-BHMT Antibody, clone 8C11H5: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: BHMT UniProt Number: Q93088
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MABN679
100 µL  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN679
      ReplacesMABE536
      DescriptionAnti-BHMT Antibody, clone 8C11H5
      Alternate Names
      • Betaine--homocysteine S-methyltransferase 1
      Background InformationBetaine, (trimethylglycine) is an organic osmolyte that is available for cells by either choline oxidase-catalyzed synthesis or concentrative uptake by the betaine/GABA transport system (BGT-1). The intracellular accumulation of betaine protects cells from different kinds of stress including long-lasting dehydration, ischemia-reperfusion, and heat shock. Betaine has been considered a “chemical chaperone” because it prevents protein denaturation under adverse conditions, a property that likely contributes to the cytoprotective effects of this osmolyte.
      Betaine--homocysteine S-methyltransferase 1, or betaine-homocysteine methyltransferase (BHMT) is the enzyme that is responsible for the metabolic elimination of betaine and homocysteine via their conversion to dimethylglycine and methionine. As such BHMT is one of a handful of key enzymes keenly responsible for betaine levels. BHMT is expressed at high levels in the human liver and kidney, localizing in the ER and cytoplasmic regions. BHMT expression is largely regulated at the level of the gene. BHMT activity becomes essential when folate-dependent remethylation is impaired because of nutritional deficiencies as it then provides the source for methionine. Deficiencies in BHMT can lead a variety of disorders ranging from vascular disease to neural tube birth defects such as spina bifida, fatty liver disease and cirrhosis all which can be linked to betaine metabolism disorders. EMD-Millipore’s Anti-BHMT mouse monoclonal antibody, clone 635, has been tested in western blots using recombinantly expressed BHMT cDNA and by immunohistochemistry on paraffin embedded human liver tissue samples.
      References
      Product Information
      FormatAscites
      Control
      • HepG2 cell lysate
      PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-BHMT Antibody, clone 8C11H5 is a highly specific mouse monoclonal antibody, that targets Betaine--homocysteine S-methyltransferase 1 & has been tested in IHC & western blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: 1:500-1,000 dilution from a representative lot detected BHMT in human kidney and liver tissues.
      Biological Information
      ImmunogenPurified recombinant fragment of BHMT expressed in E. coli.
      Clone8C11H5
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BHMT
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~50 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected BHMT in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABN679 04053252910265