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ST1049 Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subuni

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ST1049
  
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      Descripción

      Tabla espec. clave

      Species ReactivityHostAntibody Type
      H, M, Rb, R, YeastMMonoclonal Antibody
      Description
      OverviewRecognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
      Catalogue NumberST1049
      Brand Family Calbiochem®
      Application Data
      Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
      References
      ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHuman placental tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunofluorescence
      Immunohistochemistry
      Not Immunoprecipitation
      Application NotesImmunoblotting (1:1000, chemiluminescence)
      Immunoprecipitation (not recommended)
      ELISA (see comments)
      Immunofluorescence (see application references)
      Immunocytochemistry (see application references)
      Immunohistochemistry (see application references)
      Frozen Sections (see application references)
      Application CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogendinitrophenylated proteasomes
      ImmunogenHuman
      Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
      CloneMCP231
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rabbit
      • Rat
      • Yeast
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Número de referencia GTIN
      ST1049 0