Millipore Sigma Vibrant Logo

176900 Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
View Products on Sigmaaldrich.com
176900
  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, Bacillus anthracis anthrax lethal factor expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, lethal factor (LF) is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains. Domain I binds the protective antigen to enter the target cell; domains II, III, and IV create a long groove to hold and cleave the MAPKK proteins.
      Catalogue Number176900
      Brand Family Calbiochem®
      References
      ReferencesBrossier, F., and Mock, M. 2001. Toxicon 39, 1747.
      Friedlander, A.M. 2001. Nature 414, 160.
      Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun. 284, 568.
      Pannifer, A.D., et al. 2001. Nature 414, 160.
      Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA 93, 8155.
      Leppla, S.H. 1991. Methods Enzymol. 195, 153.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to catalyze the release of 1.0 pmole of cleaved MAPKKide™ substrate per min at 37°C, in 20 mM HEPES, pH 8.2.
      FormLyophilized
      FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetMAPKK
      PuritySingle major band by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
      End use certificateY
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      176900 0

      Documentation

      Licencias necesarias

      Título
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anthrax Lethal Factor, Recombinant, Bacillus anthracis, B. anthracis - Calbiochem Certificados de análisis

      CargoNúmero de lote
      176900

      Referencias bibliográficas

      Visión general referencias
      Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.
      Friedlander, A.M. 2001. Nature 414, 160.
      Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun. 284, 568.
      Pannifer, A.D., et al. 2001. Nature 414, 160.
      Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA 93, 8155.
      Leppla, S.H. 1991. Methods Enzymol. 195, 153.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-October-2013 JSW
      DescriptionRecombinant, Bacillus anthracis anthrax lethal factor expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Highly specific protease component that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains: domain I that binds to protective antigen (necessary for entry into the cellular target) and domains II, III, and IV which form a long groove to hold and cleave the target proteins. Requires passage through an acidic vesicle prior to translocation to the cytoplasm of the cell. Cleavage of target proteins leads to the inhibition of multiple signaling pathways and cell death.
      FormLyophilized
      FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
      PuritySingle major band by SDS-PAGE
      Unit definitionOne unit is defined as the amount of enzyme required to catalyze the release of 1.0 pmole of cleaved MAPKKide™ substrate per min at 37°C, in 20 mM HEPES, pH 8.2.
      SolubilityPlease refer to the vial label for lot-specific reconstitution volume.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesBrossier, F., and Mock, M. 2001. Toxicon 39, 1747.
      Friedlander, A.M. 2001. Nature 414, 160.
      Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun. 284, 568.
      Pannifer, A.D., et al. 2001. Nature 414, 160.
      Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA 93, 8155.
      Leppla, S.H. 1991. Methods Enzymol. 195, 153.