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17-128 Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A)

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17-128
1 kit  Kit capacity: 100 assays
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key ApplicationsAnalytes AvailableDetection Methods
      PAAlkaline/Acid Chromogenic
      Description
      Catalogue Number17-128
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAlkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A)
      References
      Product Information
      Components
      • Malachite Green Solution A (Cat.# 20-105)
      • Malachite Green Additive (Cat.# 20-104)
      • Phosphate Standard (Cat.# 20-103)
      • Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)
      • pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
      • NiCl2, 40mM (Cat.# 20-178)
      • pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
      • 96-well microtiter plate
      Detection methodChromogenic
      HS Code3002 15 90
      Quality LevelMQ100
      Applications
      ApplicationAlkaline/Acid Phosphatase Assay Kit is routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP.
      Key Applications
      • Phosphatase Assay
      Biological Information
      Analytes Available
      • Alkaline/Acid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 100 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      17-128 04053252475177

      Documentation

      Licencias necesarias

      Título
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) Certificados de análisis

      CargoNúmero de lote
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) - 2119192 2119192
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) - 2383493 2383493
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) - 2148453 2148453
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) 2936927
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) 2975540
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) 2931212
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) 3102619
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) - 12811 12811
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) - 18014 18014
      Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A) - 20944 20944

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides
      Harder, K. W., et al
      Biochem J, 298 ( Pt 2):395-401 (1994)  1993

      Phosphatase Assay8135747 8135747
      Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green.
      Ekman, P and Jäger, O
      Anal. Biochem., 214: 138-41 (1993)  1992

      Mostrar resumen
      Phosphatase Assay8250216 8250216
      The use of phosphopeptides to distinguish between protein phosphatase and acid/alkaline phosphatase activities: opposite specificity toward phosphoseryl/phosphothreonyl substrates.
      Donella-Deana, A, et al.
      Biochim. Biophys. Acta, 1094: 130-3 (1991)  1991

      Mostrar resumen
      1653021 1653021
      An investigation of the substrate specificity of protein phosphatase 2C using synthetic peptide substrates; comparison with protein phosphatase 2A
      Donella Deana, A., et al
      Biochim Biophys Acta, 1051:199-202 (1990)  1990

      Phosphatase Assay2155667 2155667
      Inorganic and organic phosphate measurements in the nanomolar range.
      Van Veldhoven, P P and Mannaerts, G P
      Anal. Biochem., 161: 45-8 (1987)  1987

      Mostrar resumen
      Phosphatase Assay3578786 3578786

      Preguntas frecuentes

      PreguntaRespuesta
      I still have material left to run more assays, but I need another plate. Where can I find a comparable plate?To get a comparable plate, you can purchase a Costar Brand 96-Well Half Area Plate (for Tissue Culture Section).

      Productos y aplicaciones relacionados

      Kit Component

      Número de referencia Descripción  
      12-220 Serine Phosphopeptide (RRApSVA) Precios y disponibilidad
      20-103 Phosphate Standard Precios y disponibilidad
      20-104 Malachite Green Additive Precios y disponibilidad
      20-105 Malachite Green Solution A Precios y disponibilidad
      20-106 pNPP (p-Nitrophenyl Phosphate) Precios y disponibilidad
      20-179 pNPP Ser/Thr Assay Buffer Precios y disponibilidad

      Categorías

      Life Science Research > Protein Detection and Quantification > Activity Assays > Phosphatase Assays