AAMT009 Sigma-AldrichAconitase Activity Assay Kit

This product has been discontinued.

Product Description

The active form of the enzyme is inhibited by citrate analogs, and fluoracetate. Other inhibitors include oxidative stress agents such as peroxynitrite, hydrogen peroxide and superoxide, which inactivate the enzyme by changing the [4Fe-4S] to a [3Fe-4S] cluster. Therefore, aconitase is considered a good marker of mitochondrial and cellular oxidative stress. This inhibition of mitochondrial aconitase can lead to a decreased energy production, whereas in cytosolic aconitase it triggers binding of the enzyme to mRNA iron response elements resulting in increased expression of iron uptake proteins and decreased transcription of iron sequestering protein.

A hydroxyl scavenging solution (Aconitase preservation solution) is supplied with this kit to maintain aconitase activity during sample preparation. An inactivated [3Fe-4FS] aconitase may be activated in vitro by the addition of iron and cysteine.

50 ml 	Buffer (Tube 1), (72575-50ML)
20 ml	Aconitase preservation solution, (72576-20ML)
800 µl  	25X Isocitrate, (72577-800UL)
200 µl	100X Manganese, (72578-200UL)
1 ea	UV Microplate, (72579-1EA)
1 ml	Detergent, (72580-1ML)
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).