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SCM112 3dGRO™ Wnt3a Conditioned Media Supplement

Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.

SCM112
50 mL  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberSCM112
      Description3dGRO™ Wnt3a Conditioned Media Supplement
      Alternate Names
      • Wnt3a Conditioned Media
      • Wnt3a CM
      • Wnt Conditioned Media
      Background Information3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. Wnt proteins hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin resulting in expression of genes regulating cell development. Wnt3a is considered an essential protein in many organoid culture systems (intestinal, pancreatic, liver, colon etc.) because the proliferation of stem cells is governed by the Wnt signaling pathway. However, due to the high concentration requirement in organoid media, the use of the recombinant Wnt3a becomes prohibitively expensive. Additionally, recombinant Wnt3a proteins are often unstable and lack biological activity. The 3dGRO™ Wnt3a Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant Wnt3a protein for organoid cultures and is optimized for the highest level of Wnt3a expression to ensure consistency and performance.

      Note: Product is supplied at a 5X concentration (see datasheet for a complete organoid media recipe).

      Source
      The 3dGRO™ Wnt3a Conditioned Media Supplement is generated from mouse L-M(TK-) cells transfected with a mouse Wnt-3A expression vector. The conditioned medium contains fetal bovine serum.
      References
      Product Information
      Applications
      ApplicationAlternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Biological Information
      Gene Symbol
      • WNT3A
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Appearance (color): clear, red liquid
      • Endotoxin: <2 EU/mL
      • Sterility Tested: No Growth/Pass
      • Mycoplasma: Negative
      • Media tested for function in supporting growth of human pancreatic organoids
      • Media tested for biological activity using a Wnt3a luciferase reporter assay in HEK293 STF cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWnt3a Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed Wnt3a Conditioned Medium is good for two weeks when stored at 2-8°C.
      Packaging Information
      Material Size50 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      SCM112 04065269237051

      Documentation