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344826 α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli

This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: α-L-Fucoside Fucohydrolase

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344826
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Precios y disponibilidad

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344826-50MIU
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      Description
      OverviewRecombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
      Note: 1 mU = 1 milliunit.
      Catalogue Number344826
      Brand Family Calbiochem®
      Synonymsα-L-Fucoside Fucohydrolase
      References
      ReferencesZheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.
      Product Information
      CAS number9037-65-4
      Activity≥1.0 unit/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
      EC number3.2.1.51
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥1.5 units/mg protein
      Physicochemical Information
      ContaminantsProteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      344826-50MIU 04055977214826

      Documentation

      α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli Certificados de análisis

      CargoNúmero de lote
      344826

      Referencias bibliográficas

      Visión general referencias
      Zheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-October-2007 JSW
      Synonymsα-L-Fucoside Fucohydrolase
      DescriptionRecombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium meningosepticum) catalyses the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions
      50 mM sodium phosphate buffer, pH 5.0.
      CAS number9037-65-4
      EC number3.2.1.51
      ContaminantsProteases: none detected
      Specific activity≥1.5 units/mg protein
      Activity≥1.0 unit/ml
      Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesZheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.