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71228 pSTBlue-1 AccepTor™ Vector Giga Kit - Novagen

Featuring super-efficient competent cells for increased transformation efficiency

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71228
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Overview

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71228-3
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      71228-4
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          Description
          Overview

          The pSTBlue-1 AccepTor™ Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.

          The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.
          Catalogue Number71228
          Brand Family Novagen®
          Features and benefits• No restriction enzymes or special primers
          • Direct ligation of PCR product with vector
          • Compatible with polymerases that leave single 3´-dA overhangs
          • Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
          • Simple protocol takes as little as 40 minutes from PCR product to plating transformants
          Product Information
          Components
          DeclarationThis product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates.
          Quality LevelMQ100
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Global Trade Item Number
          Catalogue Number GTIN
          71228-3 04055977271133
          71228-4 04055977271140