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70759 pET Expression System 19b - Novagen

70759
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      pET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 19b
      The pET-19b vector carries an N-terminal His•Tag® sequence followed by an enterokinase cleavage site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB049VM.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      Catalogue Number70759
      Brand Family Novagen®
      References
      Product Information
      Components
      Fusion tagHis•Tag
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70759 0

      Documentation

      pET Expression System 19b - Novagen Certificates of Analysis

      TitleLot Number
      70759

      Citations

      Title
    • Barbara Becattini, et al. (2006) Structure-activity relationships by interligand NOE-based design and synthesis of antiapoptotic compounds targeting Bid. Procedings of the National Academy of Science 103, 12602-12606.
    • Andrew D. Ferguson, et al. (2006) NMR structures of the selenoproteins sep15 and selM reveal redox activity of a new thioredoxin-like family. Journal of Biological Chemistry 281, 3536-3543.
    • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
    • Micah M. Bhatti and William J. Sullivan, Jr.. (2005) Histone acetylase GCN5 enters the nucleus via importin- in protozoan parasite Toxoplasma gondii. Journal of Biological Chemistry 280, 5902-5908.
    • Marcelo Comini, et al. (2005) Trypanothione synthesis in Crithidia revisited. Journal of Biological Chemistry 280, 6850-6860.
    • Eric S. Folker, Brian M. Baker and Holly V. Goodson. (2005) Interactions between CLIP-170, tubulin, and microtubules: implications for the mechanism of CLIP-170 plus-end tracking behavior. Molecular Biology of the Cell 16, 5373-5384.
    • Antony M. Jose and Michael R. Koelle. (2005) Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivo. Journal of Biological Chemistry 280, 2730-2736.
    • Vyacheslav M. Labunskyy, et al. (2005) A novel cysteine-rich domain of SEP15 mediates the interactions with UDP-glucose: Glycoprotein glucosyltransferase. Journal of Biological Chemistry 280, 37839-37845.
    • Rory E. Morty, et al. (2005) Oligopeptidase B from Trypanosoma evansi: a parasite peptidase that inactivates atrial natriuretic factor in the bloodstream of infected hosts. Journal of Biological Chemistry 280, 10925-10937.
    • Belinda J. Hales, et al. (2004) Isoforms of the major peanut allergen arah2: IgE binding in children with peanut allergy. International Archives of Allergy and Immunology 135, 101-107.
    • Yuhong Wang, et al. (2004) Human myoglobin recognition of oxygen: dynamics of the energy landscape. Procedings of the National Academy of Science 101, 18000-18005.
    • Michael Albers, et al. (2003) Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors. RNA 9, 138-150.
    • Edward J. Kim and David M. Helfman. (2003) Characterization of the metastasis-associated protein, S100A4. Journal of Biological Chemistry 278, 30063-30073.
    • G. Chang, et al. (1998) Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282, 2220-2226.
    • User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB049VM pET-19b Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors