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70791 pET Dsb Fusion System 40b

Vectors for export and periplasmic folding of target proteins

pET Dsb Fusion System 40b MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70791
  
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      Overview

      Description
      Overview

      This product has been discontinued.





      The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)].

      DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).








      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      Catalogue Number70791
      Brand Family Novagen®
      References
      References

      Rietsch, A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13048. Sone, M., et al. 1997. J. Biol. Chem. 272, 10349. Missiakas, D., et al. 1994. EMBO J. 13, 2013. Zapun, A., et al. 1995. Biochemistry 34, 5075. Raina, S. and Missiakas, D. 1997. Ann. Rev. Microbiol. 51, 179. Collins-Racie, L.A., et al. 1995. Bio/Technology 13, 982.
      Product Information
      Components
      Fusion tagHis•Tag, S•Tag
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      70791 0