71251 Sigma-AldrichVeggie NovaBlue Singles™ Competent Cells - Novagen
Recommended Products
Overview
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Pricing & Availability
| Catalogue Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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| 71251-3 |
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Plastic ampoule | 11 rxn |
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| 71251-4 |
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Glass bottle | 22 rxn |
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| Description | |
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| Overview | Veggie™ NovaBlue Singles™ Competent Cells are available for routine cloning applications. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and mutations in recA and endA, which result in high yields of high-quality plasmid DNA. |
| Genotype:
endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
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| Catalogue Number | 71251 |
| Brand Family | Novagen® |
| Features and benefits | • Guaranteed efficiency > 1.5 × 10⁸ cfu/µg • Manufactured free of animal-derived media and components • Reproducible High-efficiency cloning • Produces high-quality plasmid DNA • Easy-to-use Singles™ format • Prepared using an optimized chemical method |
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| Product Information | ||
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| • | 11 or 22 rxn | Veggie NovaBlue Singles Competent Cells |
| • | 2 or 4 × 2 ml | SOC Medium |
| • | 10 µl | Test Plasmid |
| Genotype | endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA+B+ lacIqZΔM15::Tn10] (TetR) | |
| Quality Level | MQ100 | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 71251-3 | 04055977270815 |
| 71251-4 | 04055977270822 |
Documentation
Veggie NovaBlue Singles™ Competent Cells - Novagen Certificates of Analysis
| Title | Lot Number |
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| 71251 |
Brochure
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| High fidelity gene amplification |
| The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression |
User Protocols
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| TB358 Veggie™ Singles™ Competent Cells |