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676472 VEGF₁₆₅, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Vascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin

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676472
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.



      Recombinant, human VEGF165 (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic, and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfide-linked homodimer.
      Catalogue Number676472
      Brand Family Calbiochem®
      SynonymsVascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin
      References
      ReferencesLiu, X., et al. 2001. J. Urology 166, 354.
      Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
      Neufeld, G., et al. 1994. Prog. Growth Factor Res. 5, 89.
      Leung, D.W., et al. 1989. Science 246, 1306.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg VEGF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      676472 0

      Documentation

      VEGF₁₆₅, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      VEGF₁₆₅, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      676472

      References

      Reference overview
      Liu, X., et al. 2001. J. Urology 166, 354.
      Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
      Neufeld, G., et al. 1994. Prog. Growth Factor Res. 5, 89.
      Leung, D.W., et al. 1989. Science 246, 1306.

      Citations

      Title
    • Diane Gesty-Palmer, et al. (2005) b-Arrestin 2 expression determines the transcriptional response to lysophosphatidic acid stimulation in murine embryo fibroblasts. Journal of Biological Chemistry 280, 32157-32167.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsVascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin
      DescriptionRecombinant, human VEGF165 (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfied-linked homodimer.
      FormLyophilized
      FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg VEGF
      Biological activityED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells
      SolubilityReconstitute to a final concentration of ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.
      Toxicity Standard Handling
      ReferencesLiu, X., et al. 2001. J. Urology 166, 354.
      Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
      Neufeld, G., et al. 1994. Prog. Growth Factor Res. 5, 89.
      Leung, D.W., et al. 1989. Science 246, 1306.
      Citation
    • Diane Gesty-Palmer, et al. (2005) b-Arrestin 2 expression determines the transcriptional response to lysophosphatidic acid stimulation in murine embryo fibroblasts. Journal of Biological Chemistry 280, 32157-32167.
      		•