Millipore Sigma Vibrant Logo

650200 Trypsin, Bovine Pancreas, High Purity, Endotoxin-Free

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
650200
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.

      We are offering Trypsin, Bovine Pancreas (Cat. No. 6502) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.





      Native trypsin highly purified from bovine pancreas. Endotoxins removed chromatographically. Serine protease which specifically hydrolyzes peptide bonds at the C-terminus side of arginine and lysine residues. May be used for cell harvesting as well as tissue dissociation when combined with elastase and/or collagenase.
      Catalogue Number650200
      Brand Family Calbiochem®
      References
      ReferencesNishino, N., et al. 1974. J. Biochem. (Tokyo) 75, 979.
      Brown, W., and Wold, F. 1973. Biochemistry 12, 828.
      Schroeder, D., et al. 1968. J. Biol. Chem. 243, 2943.
      Product Information
      CAS number9002-07-7
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze BAEE to cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
      EC number3.4.21.4
      FormLiquid
      Formulation5 mg per ml in 0.9% sodium chloride. Sterile-filtered.
      Applications
      Biological Information
      Purity≥90% by HPLC (gel filtration)
      Specific Activity≥3000 USP units/mg protein
      Physicochemical Information
      ContaminantsChymotrypsin: ≤40 NF units/mg protein; endotoxins ≤8 EU/mg
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSYN5075000
      Safety Information
      R PhraseR: 36/37/38-42

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation.
      S PhraseS: 24-26-36/37

      Avoid contact with skin.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing and gloves.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      650200 0

      Documentation

      Trypsin, Bovine Pancreas, High Purity, Endotoxin-Free SDS

      Title

      Safety Data Sheet (SDS) 

      Trypsin, Bovine Pancreas, High Purity, Endotoxin-Free Certificates of Analysis

      TitleLot Number
      650200

      References

      Reference overview
      Nishino, N., et al. 1974. J. Biochem. (Tokyo) 75, 979.
      Brown, W., and Wold, F. 1973. Biochemistry 12, 828.
      Schroeder, D., et al. 1968. J. Biol. Chem. 243, 2943.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-May-2008 RFH
      DescriptionNative trypsin highly purified from bovine pancreas. A serine protease that specifically hydrolyzes peptide bonds at the C-terminus side of arginine and lysine residues. May be used for cell harvesting as well as tissue dissociation when combined with elastase and/or collagenase. Endotoxins were removed chromatographically.
      FormLiquid
      Formulation5 mg per ml in 0.9% sodium chloride. Sterile-filtered.
      CAS number9002-07-7
      RTECSYN5075000
      EC number3.4.21.4
      Purity≥90% by HPLC (gel filtration)
      ContaminantsChymotrypsin: ≤40 NF units/mg protein; endotoxins ≤8 EU/mg
      Specific activity≥3000 USP units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze BAEE to cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      Merck USA index14, 9795
      ReferencesNishino, N., et al. 1974. J. Biochem. (Tokyo) 75, 979.
      Brown, W., and Wold, F. 1973. Biochemistry 12, 828.
      Schroeder, D., et al. 1968. J. Biol. Chem. 243, 2943.