Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Native thermolysin from Geobacillus stearothermophilus. Zinc metalloendoproteinase with specificity toward isoleucine, leucine, methionine and valine. Inhibitors include EDTA, 1,10-phenanthroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Note: 1 KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number
58656
Brand Family
Calbiochem®
References
References
Pfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523. Matsubara, H. 1970. Methods Enzymol. 19, 642. Ohta, Y., et al. 1966. J. Biol. Chem.241, 5919.
Product Information
CAS number
37246-64-3
Activity
≥7,000,000 PU/g dry weight
PU = protease unit
Unit of Definition
One protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
Thermolysin, Geobacillus stearothermophilus Certificates of Analysis
Title
Lot Number
58656
References
Reference overview
Pfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523. Matsubara, H. 1970. Methods Enzymol. 19, 642. Ohta, Y., et al. 1966. J. Biol. Chem.241, 5919.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-June-2015 JSW
Description
Native thermolysin from Geobacillus stearothermophilus. A highly thermostable zinc metallo-endoproteinase that exhibits specificity toward isoleucine, leucine, methionine, and valine. Inhibitors include EDTA, 1,10-phenathroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Has an optimal pH in the range 7.0-9.0.
Please refer to vial label for lot-specific concentration
CAS number
37246-64-3
RTECS
XH7500000
EC number
3.4.24.2
Activity
≥7,000,000 PU/g dry weight
PU = protease unit
Unit definition
One protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
Solubility
Reconstitute in 42% glycerol adjusted to pH 8.0 with 0.01 N NaOH, containing 0.005% Triton® X-100 detergent.
Storage
+2°C to +8°C Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity
Harmful
Merck USA index
14, 9286
References
Pfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523. Matsubara, H. 1970. Methods Enzymol. 19, 642. Ohta, Y., et al. 1966. J. Biol. Chem.241, 5919.
