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575305 T0070907 - CAS 313516-66-4 - Calbiochem

A cell-permeable chloro-nitro-benzamido compound that acts as a potent, specific, irreversible, and high-affinity antagonist of PPARγ with a Ki of 1 nM.

T0070907 - CAS 313516-66-4 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 2-Chloro-5-nitro-N-(4-pyridyl)benzamide

Primary Target: PPARγ
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      313516-66-4C₁₂H₈ClN₃O₃
      Description
      OverviewA cell-permeable chloro-nitro-benzamido compound that acts as a potent, specific, irreversible, and high-affinity antagonist of PPARγ with a Ki of 1 nM. Displays >800-fold greater selectivity for PPARγ over PPARα and PPARδ (Ki = 0.85 µM and 1.8 µM, respectively). Blocks hormone- and agonist-induced adipogenesis in 3T3-L1 cells. It suppresses interactions between PPARγ and coactivator-derived peptides, while promotes the recruitment of corepressor-derived peptides. Shown to modulate the interaction of PPARγ2 with the cofactor proteins through covalent binding to Cys313 in its ligand-binding domain.
      Catalogue Number575305
      Brand Family Calbiochem®
      Synonyms2-Chloro-5-nitro-N-(4-pyridyl)benzamide
      References
      ReferencesLee, G., et al. 2002. J. Biol. Chem. 277, 19649.
      Product Information
      CAS number313516-66-4
      ATP CompetitiveN
      FormWhite solid
      Hill FormulaC₁₂H₈ClN₃O₃
      Chemical formulaC₁₂H₈ClN₃O₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetPPARγ
      Primary Target K<sub>i</sub>1 nM for PPARγ
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Carcinogenic / Teratogenic
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      575305 0

      Documentation

      T0070907 - CAS 313516-66-4 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      T0070907 - CAS 313516-66-4 - Calbiochem Certificates of Analysis

      TitleLot Number
      575305

      References

      Reference overview
      Lee, G., et al. 2002. J. Biol. Chem. 277, 19649.

      Citations

      Title
    • Iwona Bogacka, et al. (2005) Structural and functional consequences of mitochondrial biogenesis in human adipocytes in vitro. Journal of Clinical Endocrinology and Metabolism in press,.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-September-2008 RFH
      Synonyms2-Chloro-5-nitro-N-(4-pyridyl)benzamide
      DescriptionA cell-permeable, potent, specific, irreversible, and high-affinity antagonist of PPARγ (Ki = 1 nM). Reported to be >800-fold more specific for PPARγ compared to PPARα (Ki = 850 nM) and PPARδ (Ki = 1.8 µM). Shown to block hormone- and agonist-induced adipogenesis in 3T3-L1 cells. Suppresses interactions between PPARγ and coactivator-derived peptides while promoting the recruitment of co-repressor-derived peptides. Also shown to modulate the interaction of PPARγ2 with cofactor proteins through covalent binding to Cys313 in the ligand-binding domain.
      FormWhite solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number313516-66-4
      Chemical formulaC₁₂H₈ClN₃O₃
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Carcinogenic / Teratogenic
      ReferencesLee, G., et al. 2002. J. Biol. Chem. 277, 19649.
      Citation
    • Iwona Bogacka, et al. (2005) Structural and functional consequences of mitochondrial biogenesis in human adipocytes in vitro. Journal of Clinical Endocrinology and Metabolism in press,.
      		•