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115948 Safranine O (C.I. 50240)

for microscopy Certistain®

Safranine O (C.I. 50240) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CAS #: 477-73-6 EC Number: 207-518-8 Molar Mass: 350.85 g/mol Hill Formula: C₂₀H₁₉ClN₄
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115948
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Overview

Replacement Information

Key Spec Table

CAS #EC NumberHill FormulaMolar Mass
477-73-6207-518-8C₂₀H₁₉ClN₄350.85 g/mol
Description
Catalogue Number115948
DescriptionSafranine O (C.I. 50240)
OverviewSafranin O (C.I. 50240) for microscopy Certistain®, is a dry dye that is used for the preparation of a staining solution for e.g. Gram stain procedures in smears and sections of human origin in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Gram-negative bacteria will be counterstained by Gram's safranin O solution and appear orange. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
CAS number477-73-6
EC number207-518-8
Hill FormulaC₂₀H₁₉ClN₄
Molar Mass350.85 g/mol
HS Code3212 90 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
Applicationfor microscopy Certistain®
Biological Information
Physicochemical Information
pH value10 (10 g/l, H₂O, 20 °C)
Bulk density400 kg/m3
Solubility50 g/l
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H318: Causes serious eye damage.
Precautionary Statement(s)P280: Wear eye protection/ face protection.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal WordDanger
RTECSSG1623000
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Dye content (spectrophotometrically)≥ 80 %
Identity (UV/VIS-Spectrum)passes test
Absorption maximum λ max (ethanol 50 %)530 - 534 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)1260 - 1570
TLC-Testpasses test
Loss on drying (110 °C)≤ 15 %
Suitability for microscopypasses test