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HTS241RTA Ready-to-Assay™ SUCNR1 / GPR91 Succinate Receptor Frozen Cells

Human SUCNR1 / GPR91 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_033050
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HTS241RTA
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      Overview

      Replacement Information

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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1SUCNR1 / GPR91
      Description
      Catalogue NumberHTS241RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ SUCNR1 / GPR91 Succinate Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      SUCNR1, or GPR91, is a class A G-protein coupled receptor that binds selectively to succinate, an intermediate in the citric acid cycle, and couples to both the Gi/Go and Gq pathways. Renal proximal tubules are a major site of GPR91 expression, and succinate-mediated activation of renal GPR91 stimulates the release of renin to cause vasoconstriction and hypertension (He et al., 2004). In addition, GPR91 in retinal neurons mediates retinal vascularization induced by succinate, which accumulates in response to hypoxia (Sapieha et al., 2008). Dendritic cells also highly express GPR91, which mediates succinate-induced immune cell migration and activation in vitro and in vivo (Rubic et al., 2008). Therefore, suppression of GPR91 is a potential strategy for treatment of vascular retinopathies, hypertension, and inflammation. Millipore’s cloned human GPR91 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR91 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at GPR91.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationHuman SUCNR1 / GPR91 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-1
      Protein TargetSUCNR1 / GPR91
      Target Sub-FamilySuccinate
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      HTS241RTA 04053252375224

      Documentation

      Ready-to-Assay™ SUCNR1 / GPR91 Succinate Receptor Frozen Cells SDS

      Title

      Safety Data Sheet (SDS) 

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells