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553504 RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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553504
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Overview

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553504-10KU
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      Description
      OverviewRecombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. The Pneumocystis cariniiRAD51 is equivalent to bacterial RecA and the homolog of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
      Catalogue Number553504
      Brand Family Calbiochem®
      References
      ReferencesWest, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435.
      Jasin, M. 2002. Oncogene 21, 8981.
      Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630.
      Daboussi, F., et al. 2002. Cell. Signal. 14, 969.
      Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.
      Product Information
      Unit of DefinitionOne unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
      FormLiquid
      FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      553504-10KU 04055977193657

      Documentation

      RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      553504

      References

      Reference overview
      West, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435.
      Jasin, M. 2002. Oncogene 21, 8981.
      Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630.
      Daboussi, F., et al. 2002. Cell. Signal. 14, 969.
      Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-April-2016 JSW
      DescriptionRecombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
      FormLiquid
      FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Unit definitionOne unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesWest, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435.
      Jasin, M. 2002. Oncogene 21, 8981.
      Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630.
      Daboussi, F., et al. 2002. Cell. Signal. 14, 969.
      Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.