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325540 Protein Tyrosine Kinase Positive Control, Rat Spleen Extract

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
325540
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Has been validated for use as a positive control for protein tyrosine kinase (PTK) activity assays. There is sufficient PTK activity at a concentration of 10-20 ng/well using the K-LISA™ PTK Screening Kit (Cat. No. 539701).
      Catalogue Number325540
      Brand Family Calbiochem®
      Application Data
      The rat spleen extract was reconstituted as indicated above, diluted in reaction buffer, and assayed using the K-LISA™ PTK Screening Kit according to the recommended protocol. Absorbance values shown represent the mean of triplicate samples that were corrected against background absorbance. Background absorbance values ranged from ~0.05-0.07 absorbance units.
      References
      ReferencesElberg, G. et al., 1995. Biochim. Biophys. Acta. 1269, 299.
      Swarup, G. et al. 1983. J. Biol. Chem. 258, 10341.
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      325540 0

      Documentation

      References

      Reference overview
      Elberg, G. et al., 1995. Biochim. Biophys. Acta. 1269, 299.
      Swarup, G. et al. 1983. J. Biol. Chem. 258, 10341.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-September-2008 JSW
      Application Data
      The rat spleen extract was reconstituted as indicated above, diluted in reaction buffer, and assayed using the K-LISA™ PTK Screening Kit according to the recommended protocol. Absorbance values shown represent the mean of triplicate samples that were corrected against background absorbance. Background absorbance values ranged from ~0.05-0.07 absorbance units.
      DescriptionThe Protein Tyrosine Kinase Positive Control, Rat Spleen Extract, 7 mg has been validated for use as a positive control for protein tyrosine kinase (PTK) activity assays. There is sufficient PTK activity at a concentration of 10-20 ng/well using the K-LISA™ PTK Screening Kit (Cat. No. 539701).

      Assay Characteristics and Examples

      The assay example below represents typical data obtained using the rat spleen extract in a tyrosine kinase assay. Actual tyrosine kinase activity may vary from lot to lot.
      Recommended reaction conditionsReagent Preparation Reconstitute the entire vial of rat spleen extract with 250 µl of ultra pure H2O. The resulting suspension has a concentration of 28 mg/ml total protein. Dispense into aliquots and store at -70°C. Use one aliquot as positive control for tyrosine kinase assays. There is sufficient PTK activity at 10-20 ng/well to serve as a strong positive control (see example below). Discard the unused portion; do not re-freeze.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesElberg, G. et al., 1995. Biochim. Biophys. Acta. 1269, 299.
      Swarup, G. et al. 1983. J. Biol. Chem. 258, 10341.