Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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539253
Sigma-AldrichProtein A, Peroxidase Conjugate
This Protein A, Peroxidase Conjugate is validated for use in Direct Immunoassay.
More>>This Protein A, Peroxidase Conjugate is validated for use in Direct Immunoassay. Less<<
Protein A, Peroxidase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
o-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Purity
Single band 50,000 on SDS-PAGE
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Yes
Mercury prohibited
To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Protein A, Peroxidase Conjugate Certificates of Analysis
Title
Lot Number
539253
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
13-August-2007 RFH
Application
Direct Immunoassay (1:10,000)
Description
Native protein A from Staphylococcus aureus conjugated to horseradish peroxidase.
Background
Protein A is the universal secondary antibody because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors and therefore will not give high background reactions.
Form
Liquid
Formulation
In 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
Single band 50,000 on SDS-PAGE
Preservative
0.02% thimerosal
Comments
o-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.