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CBA009 PhosphoDetect™ p53 (pSer¹⁵) ELISA Kit

PhosphoDetect™ p53 (pSer¹⁵) ELISA Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Detection Methods: Colorimetric
CBA009
  
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      Overview

      Key Spec Table

      Detection Methods
      Colorimetric
      Description
      Overview

      This product has been discontinued.





      This product has been discontinued.



      We apologize for the inconvenience, but we do not currently have an alternative product.






      Detects p53 when phosphorylated on Ser15 in human cell lysates. Does not react with mouse or rat. p53 is phosphorylated on Ser15 by ATM, ATR, and DNA protein kinase leading to reduced interactions with its negative regulator, MDM2, and accumulation of p53.
      Catalogue NumberCBA009
      Brand Family Calbiochem®
      Materials Required but Not Delivered• Plate reader capable of measurement at or near 450 nm
      • Calibrated adjustable precision pipettes with disposable plastic tips, a manifold multi-channel pipette is helpful for large assays
      • Cell Lysis Buffer, see page 3
      • Deionized or distilled H2O
      • Automated or manual plate washer such as a squirt bottle or manifold dispenser
      • Linear, log-log, or semi-log graph paper
      • Glass or plastic tubes for diluting and aliquoting standard
      • Absorbent paper towels
      • Calibrated beakers and graduated cylinders in various sizes
      References
      ReferencesHirao, A., et al. 2000. Science 287, 1824.
      Prives, C. 1999. J. Pathol. 187, 122.
      Shieh, S.Y., et al. 1999. EMBO J. 18, 1815.
      Prokocimer, M., et al. 1998. Hum. Mutat. 12, 4.
      Steele, R.J., et al. 1998. Br. J. Surg. 85, 1460.
      Hung, J., et al. 1997. Acta Orthop. Scand. Suppl. 273, 68.
      Levine, A.J. 1997. Cell 88, 323.
      Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
      Milner, J. 1997. Pathol. Biol. (Paris) 45, 797.
      Shieh, S.Y., et al. 1997. Cell 91, 325.
      Shaw, P.H. 1996. Pathol. Res. Pract. 192, 669.
      Meek, D.W. 1994. Semin. Cancer Biol. 5, 203.
      El-Deiry, W.S., et al. 1993. Cell 75, 817.
      Lane D.P. 1992. Nature 358, 15.
      Tominaga, O., et al. 1992. Crit. Rev. Oncog. 3, 257.
      Hollstein, M., et al. 1991. Science 253, 49.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of p53 (pSer¹⁵) derived from approximately 11,000 CCRF/CEM cells treated with 5 nM actinomycin D for 20 hours.
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit containsCoated 96-Well Plate, p53 Phospho-Ser¹⁵ Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.
      Biological Information
      Assay range1-100 units/ml
      Assay time4 h
      Sample TypeCell lysates
      Physicochemical Information
      Sensitivity≤0.9 units/ml
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store the entire contents of the kit at 4°C.
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsCoated 96-Well Plate, p53 Phospho-Ser¹⁵ Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      CBA009 0