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PS1089 PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb

This PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).

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PS1089
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Pricing & Availability

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PS1089-100UL
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      Description
      OverviewRecognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
      Catalogue NumberPS1089
      Brand Family Calbiochem®
      Application Data
      Detection of human RhoA, phosphorylated at Ser188, by immunoblotting. Samples: RhoA GST fusion protein left unphosphorylated (lanes 1,3) or phosphorylated in vitro by PKA (lanes 2,4,5,6). Primary antibodies: Anti-RhoA (detecting total Rho; lanes 1,2) and PhosphoDetect™ Anti-RhoA (pSer188) (Cat. No. PS1089) (1:1000) preincubated without blocking peptide (lanes 3,4), preincubated with phosphoRhoA (pSer188) blocking peptide (lane 5), and preincubated with nonspecific phosphoserine containing blocking peptide (lane 6). Detection: chemiluminescence.
      References
      ReferencesEllerbroek, S.M., et al. 2003. J. Biol. Chem. 278, 19023.
      Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
      Dong, J.M., et al. 1998. J. Biol. Chem. 273, 22554.
      Lang, P., et al. 1996. EMBO J. 15, 510.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
      Positive controlRhoA GST fusion protein
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      ELISA (1:2000)
      Application CommentsDoes not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PS1089-100UL 04055977226577

      Documentation

      PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PS1089

      References

      Reference overview
      Ellerbroek, S.M., et al. 2003. J. Biol. Chem. 278, 19023.
      Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
      Dong, J.M., et al. 1998. J. Biol. Chem. 273, 22554.
      Lang, P., et al. 1996. EMBO J. 15, 510.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-July-2008 JSW
      ApplicationImmunoblotting (1:1000)
      ELISA (1:2000)
      Application Data
      Detection of human RhoA, phosphorylated at Ser188, by immunoblotting. Samples: RhoA GST fusion protein left unphosphorylated (lanes 1,3) or phosphorylated in vitro by PKA (lanes 2,4,5,6). Primary antibodies: Anti-RhoA (detecting total Rho; lanes 1,2) and PhosphoDetect™ Anti-RhoA (pSer188) (Cat. No. PS1089) (1:1000) preincubated without blocking peptide (lanes 3,4), preincubated with phosphoRhoA (pSer188) blocking peptide (lane 5), and preincubated with nonspecific phosphoserine containing blocking peptide (lane 6). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
      BackgroundRho (A, B, and C) proteins are members of the Ras superfamily of GTPases. They regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and the inactive GDP-bound form. Conversion from the inactive to the active form and vice versa is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The three Rho proteins are highly homologous and contain the consensus sequences necessary for GDP/GTP binding and GTPase activity. RhoA is phosphorylated at Ser188 in vitro by PKA and PKG. This phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation, indicating that Ser188 is an important site for negative regulation of RhoA activity.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
      IsotypeIgG
      Specieshuman
      Positive controlRhoA GST fusion protein
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Toxicity Standard Handling
      ReferencesEllerbroek, S.M., et al. 2003. J. Biol. Chem. 278, 19023.
      Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
      Dong, J.M., et al. 1998. J. Biol. Chem. 273, 22554.
      Lang, P., et al. 1996. EMBO J. 15, 510.