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482737 PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer¹¹⁷⁷) Mouse mAb (15E2)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Activated eNOS (pSer¹¹⁷⁷)

482737
  
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      Overview

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~140 kDa eNOS protein phosphorylated at Ser1177 in endothelial cells.
      Catalogue Number482737
      Brand Family Calbiochem®
      SynonymsAnti-Activated eNOS (pSer¹¹⁷⁷)
      Application Data
      Detection of mouse endothelial nitric oxide synthase, phosphorylated on Ser1177, by immunoblotting. Samples: Whole cell lysates from bEnd 3 cells left untreated (lane 1) and pervanadate treated (lane 2). Primary antibody: PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer1177) Mouse mAb (15E2) (Cat. No. 482737) (1 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, PEG, sucrose, 50% glycerol.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹¹⁷⁷ phosphorylation site of human endothelial nitric oxide synthase (eNOS)
      ImmunogenHuman
      Clone15E2
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      482737 0

      Documentation

      PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer¹¹⁷⁷) Mouse mAb (15E2) Certificates of Analysis

      TitleLot Number
      482737
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-May-2010 JSW
      SynonymsAnti-Activated eNOS (pSer¹¹⁷⁷)
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Application Data
      Detection of mouse endothelial nitric oxide synthase, phosphorylated on Ser1177, by immunoblotting. Samples: Whole cell lysates from bEnd 3 cells left untreated (lane 1) and pervanadate treated (lane 2). Primary antibody: PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer1177) Mouse mAb (15E2) (Cat. No. 482737) (1 µg/ml). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~140 kDa eNOS protein phosphorylated at Ser1177.
      BackgroundEndothelial NO synthase produces nitric oxide, which is crucial to cardiovascular homeostasis.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹¹⁷⁷ phosphorylation site of human endothelial nitric oxide synthase (eNOS)
      Clone15E2
      IsotypeIgG₁
      Specieshuman, mouse
      FormLiquid
      FormulationIn PBS, PEG, sucrose, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling

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      Categories

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