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382156 PhosphoDetect™ Anti-Histone H1 Rabbit pAb

PhosphoDetect™ Anti-Histone H1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
382156
  
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      Overview

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~32 kDa phosphorylated histone H1 protein. Also detects proteins at ~64 kDa and ~130 kDa.
      Catalogue Number382156
      Brand Family Calbiochem®
      References
      ReferencesPennisi, E. 1997. Science 275, 155.
      Lu, M.J., et al. 1994. Chromosoma 103, 111.
      Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.
      Product Information
      FormLiquid
      Formulation105 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Not Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunoprecipitation (not recommended)
      Application CommentsIt has not yet been determined which specific histone H1 phosphorylation sites this antibody detects. Does not cross-react with unphosphorylated histone H1. Also detects bands at ~64 kDa and ~130 kDa that have been shown to contain histone H1 using anti-histone H1 blotting. For immunocytochemistry, colcemid-treated HeLa cells were fixed with 2% paraformaldehyde followed by cold methanol. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena hyperphosphorylated isoform of Tetrahymena histone H1
      ImmunogenTetrahymena
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      382156 0