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DR1017 PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb

This PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of H2AX (pSer¹³⁹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DR1017
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form phosphorylated at Ser139.
      Catalogue NumberDR1017
      Brand Family Calbiochem®
      Application Data
      Detection of human and mouse γ-H2AX, phosphorylated at Ser139 by immunoblotting. Samples: Nuclear extract (50 µg) from HEK293 cells, G361 cells, mouse wild-type, γ-H2AX embryonic fibroblasts (+/+), and mouse γ-H2AX knockout embryonic fibroblasts (-/-), all untreated (UT) or treated with neocarzinostatin (NCS) (200 ng/ml, 30 min). Primary antibody: PhosphoDetect™ Anti-γ-H2AX (pSer139) Rabbit pAb (1:10,000). Detection: chemiluminescence.
      References
      ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
      Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
      Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
      Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
      Product Information
      FormLiquid
      FormulationIn Tris/citrate-phosphate buffer, pH 7.0-8.0.
      Positive controlHEK293 cell nuclear extract
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:5000-1:15,000)
      Immunocytochemistry (1:400-1:800)
      Application CommentsDoes not recognize the unphosphorylated form. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹³⁹ phosphorylation site of human H2AX
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1017 0

      Documentation

      PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-H2AX (pSer¹³⁹) Rabbit pAb Certificates of Analysis

      TitleLot Number
      DR1017

      References

      Reference overview
      Stiff. T., et al. 2004. Cancer Res. 64, 2390.
      Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
      Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
      Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunoblotting (1:5000-1:15,000)
      Immunocytochemistry (1:400-1:800)
      Application Data
      Detection of human and mouse γ-H2AX, phosphorylated at Ser139 by immunoblotting. Samples: Nuclear extract (50 µg) from HEK293 cells, G361 cells, mouse wild-type, γ-H2AX embryonic fibroblasts (+/+), and mouse γ-H2AX knockout embryonic fibroblasts (-/-), all untreated (UT) or treated with neocarzinostatin (NCS) (200 ng/ml, 30 min). Primary antibody: PhosphoDetect™ Anti-γ-H2AX (pSer139) Rabbit pAb (1:10,000). Detection: chemiluminescence.
      DescriptionImmunoffinity purified rabbit polyclonal antibody. Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquitinated form phosphorylated at Ser139.
      BackgroundThe nucleosome is composed of ~200 bp of DNA and eight histone proteins, two from each of the four histone protein families, H4, H3, H2B, and H2A. H2A includes three subfamilies: H2A1-H2A2, H2AZ, and H2AX, which have been conserved throughout eukaryotic evolution. Phosphorylation of H2AX on serine 139 by ATM or DNA-dependent protein kinase (DNA-PK) is an early step in the response to DNA damage by ionizing radiation. Phosphorylation of H2AX is required for the accumulation of 53BP1 at DNA break sites.
      HostRabbit
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹³⁹ phosphorylation site of human H2AX
      IsotypeIgG
      Specieshuman, mouse
      Positive controlHEK293 cell nuclear extract
      FormLiquid
      FormulationIn Tris/citrate-phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize the unphosphorylated form. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Highly Toxic
      ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
      Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
      Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
      Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.