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69965 Perfect Protein™ AP Western Blot Kit

69965
  
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      Overview

      Description
      Overview

      This product has been discontinued.



      The Perfect Protein™ AP Western Blot Kit includes an S-protein AP Conjugate in addition to the Perfect Protein™ Western Markers (Cat. No. 69959). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits.


      A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
      B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.

      Catalogue Number69965
      Brand Family Novagen®
      Features and benefits• Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection.
      • S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection.
      • Can be used with colorimetric and chemiluminescent AP or HRP substrates.
      • Recombinant, unmodified markers give sharp, accurately sized bands.
      • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection.
      • Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
      Product Information
      Components
      FormLiquid
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      69965 0