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L2223 Papain solution (61.25 mg/l)

L2223
  
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      Overview

      Replacement Information
      Description
      OverviewPapain is able to hydrolyse proteins and peptides preferably those with basic amino acids. The ready-to-use preparation (61.25 mg/l) in PBS w/o Ca2+, Mg2+ is derived from papaya fruit (Carica papaya). If compared to the usual Trypsin, Papain preparation has a limited shelf life of 36 hrs at +2-8°C. After thawing, Papain should consequently be divided into aliquots and re-frozen.
      Specifications:

      Sterility: Pass
      Functional Test: Pass
      pH: 7.1-7.5
      Osmolality: 0.270-0.310 Osm/kg H2O
      Endotoxin: Pass
      Color: Colorless
      Appearance: Clear
      Additional Information:
      Application Papain solution:
      • for passage of cell cultures, supernatant is discharged
      •cells washed with 5 ml PBS (w/o Ca2+, Mg2+; Cat. No. L 1825/0)
      • supernatant is discharged
      • supply culture flasks with (depending on size) 1.0 - 2.5 ml Papain solution
      • incubate culture for 5 - 7 min (at +15-25°C) while gently moving the flask, and observe separating progress under a microscope suitable for the survey of cells
      • transfer cells into a sterile centrifugal tube and centrifuge at 300xg for 10 min
      • discharge supernatant and resuspend pellet in 15 ml culture medium
      • repeat centrifugation, resuspend pellet in medium prior to seeding it to culture flask
      Catalogue NumberL2223
      Brand Family Calbiochem®
      References
      Product Information
      Quality LevelMQ300
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      L2223 0

      Documentation