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539735 PTP1B, Human, Recombinant, E. coli

PTP1B, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Tyrosine Phosphatase 1B

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539735
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Overview

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539735-50UG
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      		 Plastic ampoule 50 μg
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      Description
      OverviewRecombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors.
      Catalogue Number539735
      Brand Family Calbiochem®
      SynonymsProtein Tyrosine Phosphatase 1B
      References
      ReferencesPuius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
      Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphopeptide substrate (Cat. No. 539737) per min at 30°C, pH 7.2 using 150 µM of substrate.
      FormLiquid
      FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.05% NP-40, pH 7.2.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥20 units/mg
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      539735-50UG 04055977194289

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      PTP1B, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      PTP1B, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      539735

      References

      Reference overview
      Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
      Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.

      Brochure

      Title
      Protein Phosphatases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-July-2010 JSW
      SynonymsProtein Tyrosine Phosphatase 1B
      DescriptionRecombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors.
      FormLiquid
      FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.05% NP-40, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Specific activity≥20 units/mg
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphopeptide substrate (Cat. No. 539737) per min at 30°C, pH 7.2 using 150 µM of substrate.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesPuius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420.
      Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.