PEGA resins
PEGA resins swell extensively in a wide range of solvents and are ideally suited for the preparation of peptide libraries, affinity purification, and on-resin enzyme assays.
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Overview
PEGA resins are hydrophilic polymers that consist of 2-acrylamidoprop-1-yl-(2-aminoprop-1-yl) polyethylene glycol800 and dimethylacylamide cross-linked with bis 2-acrylamidoprop-1-yl polyethylene glycol800 1. These supports swell extensively in a wide range of solvents, including water, DMF, DCM, THF and MeOH, and are freely permeable to macromolecules up to 35kD. These characteristics make them ideally suited for the preparation of peptide libraries, affinity purification, and on-resin enzyme assays. These properties have been exploited in the on-resin enzymatic synthesis of glycopeptides2; for determining the inhibitors of subtilisin Carlsberg3, Cruzipain4, cysteine proteases5, and matrix metalloproteinases6; and in studies on protein disulfide isomerases using fluorescence-quenched libraries7.
1. M. Meldal (1992) Tetrahedron Lett., 33, 3077.
2. M. Meldal et al. (1994) J. Chem. Soc., Chem. Commun., 1849.
3. M. Meldal et al. (1994) Proc. Natl. Acad. Sci. USA, 91, 3314.
4. M. Meldal, et al. (1998) J. Peptide Sci., 4, 83.
5. P. M. St. Hilaire, et al. (1999) J. Comb. Chem., 1, 509.
6. J. Buchardt, et al. (2000) J. Comb. Chem., 2, 624.
7. J. C. Spetzler, et al. (1998) J. Peptide Sci., 4, 128.
