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SCC494 Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line

Mouse OP9-DL4 cells express the Notch ligand Delta-like 4 and have been demonstrated to promote T cell lineage commitment in hematopoietic stem cells. These cells support the differentiation of embryonic or hematopoietic stem cells from fetal liver or bone marrow into T lymphocytes. The OP9-DL4 cell line is an essential tool for elucidating Notch signaling in cellular differentiation and the initiation of inflammatory disease states.

SCC494
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC494
      DescriptionMouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line
      Alternate Names
      • OP9-DL4
      • OP9-DL4 cell line
      • DL4 cell line
      • Delta-like 4 cell line
      • Notch ligand cell line
      Background InformationOP9-DL4 is a stable bone marrow derived stromal cell line expressing the Notch ligand- Delta like 4 (DL4) ectopically.
      Notch signaling controls multiple cell-fate decisions. Four Notch receptors (Notch 1- Notch 4) have been identified in mammals. These receptors interact with Jagged or Delta-like (DL) family members of Notch ligands. This is followed by cleavage of the intracellular domain of Notch and its subsequent translocation into the nucleus, where it binds with transcription factors and activates transcription of various downstream target genes. Notch signaling is essential in early T- cell lineage commitments. Bone marrow progenitor cells expressing constitutively-active Notch develop into CD4 and CD8 double positive T cells rather than B cells. They also play an important role in development of CD4+ and CD8+ single positive cells from double positive precursor thymocytes, in addition to development of T-cells with αβ+ TCRs.
      Of the 4 Notch receptors, Notch1 (N1) receptor signaling has been shown to be sufficient for T cell development. The delta like ligands are physiologically relevant N1 ligands. DL1 interacts with both N1 and Notch 2 (N2) to induce T- cell lineage commitment. However, DL4 interacts specifically only with N1 and supports T cell commitment and maturation both in vitro and in vivo. Moreover, results from previous binding studies show that binding between DL1 and N1 is weak as opposed to the stronger binding between DL4 and N1. Of the DL1 and DL4 delta like ligands, DL4 exhibits greater capacity to activate the Notch pathway in hematopoietic progenitor cells.

      Source
      OP9-DL4 cells were genetically modified from OP9 stromal cells derived from mouse bone marrow.


      References
      1. Immunity 2002, 17(6): 749-756.
      2. J Orthop Sci. 2005, 10(6): 589-594.
      3. Curr Opin Immunol. 2007, 19(2): 163-168.
      4. Cold Spring Harb Protoc. 2009, 2009(2): pdb.prot5156.
      References
      Product Information
      Components
      • ≥1x106 viable OP9-DL4 hDL-4-Expressing mOP9 Cells: (Catalog No. SCC494). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationMouse OP9-DL4 cells express the Notch ligand Delta-like 4 and have been demonstrated to promote T cell lineage commitment in hematopoietic stem cells. These cells support the differentiation of embryonic or hematopoietic stem cells from fetal liver or bone marrow into T lymphocytes. The OP9-DL4 cell line is an essential tool for elucidating Notch signaling in cellular differentiation and the initiation of inflammatory disease states.
      Key Applications
      • Cell Differentiation
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      HostMouse
      Cell Line Type
      • Stromal Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsOP9-DL4 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC494 04065269082668