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411507 Mouse Anti-Human IgE, Fc Fragment Specific (HP6029)

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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.
      Catalogue Number411507
      Brand Family Calbiochem®
      References
      ReferencesReimer, C.B. Monoclonal Antibody News 4, 2.
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      PreservativeNone
      Applications
      Application ReferencesCross-Reactivity, Myeloma Proteins Reimer, C.B. Monoclonal Antibody News 4, 2. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Cross-Reactivity, Chimeric Antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Application NotesELISA (see comments)
      Application CommentsAntibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: <0.01%
      Human IgG2: <0.02%
      Human IgG3: <0.01%
      Human IgG4: <0.01%
      Human IgM: <0.1%
      Human IgA: <0.1%
      Human IgE: 100%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: <0.01%
      Human IgG2: <0.01%
      Human IgG3: <0.01%
      Human IgG4: <0.01%
      Human IgM: <0.01%
      Human IgA: <0.01%
      Human IgE: 100%
      Biological Information
      CloneHP6029
      HostMouse
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      411507 0

      Documentation

      Mouse Anti-Human IgE, Fc Fragment Specific (HP6029) Certificates of Analysis

      TitleLot Number
      411507

      References

      Reference overview
      Reimer, C.B. Monoclonal Antibody News 4, 2.
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationELISA (see comments)
      DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes human IgE Fc fragment.
      BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem® technical monograph, The Human IgG Subclasses, is available free of charge.
      HostMouse
      CloneHP6029
      IsotypeIgG
      FormLiquid
      FormulationUndiluted ascites.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: <0.01%
      Human IgG2: <0.02%
      Human IgG3: <0.01%
      Human IgG4: <0.01%
      Human IgM: <0.1%
      Human IgA: <0.1%
      Human IgE: 100%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: <0.01%
      Human IgG2: <0.01%
      Human IgG3: <0.01%
      Human IgG4: <0.01%
      Human IgM: <0.01%
      Human IgA: <0.01%
      Human IgE: 100%
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesReimer, C.B. Monoclonal Antibody News 4, 2.
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Application referencesCross-Reactivity, Myeloma Proteins Reimer, C.B. Monoclonal Antibody News 4, 2. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Cross-Reactivity, Chimeric Antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.