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FCMAB269F Milli-Mark™ Anti-Integrin β1 (CD29)-FITC conjugate Antibody, clone MB1.2

This Milli-Mark Anti-Integrin β1 (CD29)-FITC conjugate Antibody, clone MB1.2 is validated for use in FC for the detection of Integrin β1 (CD29) conjugate.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Integrins Gene Symbol: ITGB1, GPIIA, OTTHUMP00000063731, OTTHUMP00000046253, OTTHUMP00000063733, VLAB, OTTHUMP00000063732, OTTHUMP00000046253, MDF2, FNRB, CD29, MSK12, VLA-BETA UniProt Number: P05556
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FCMAB269F
100 tests  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCRFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB269F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-Integrin β1 (CD29)-FITC conjugate Antibody, clone MB1.2
      Alternate Names
      • CD29 antigen
      • Fibronectin receptor subunit beta
      • Integrin VLA-4 subunit beta
      • fibronectin receptor beta subunit
      • integrin VLA-4 beta subunit
      • integrin beta 1
      • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
      Background InformationIntegrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
      References
      Product Information
      FormatFITC
      Control
      • NIH/3T3 cells
      PresentationPurified rat monoclonal IgG2a conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Milli-Mark Anti-Integrin β1 (CD29)-FITC conjugate Antibody, clone MB1.2 is validated for use in FC for the detection of Integrin β1 (CD29) conjugate.
      Key Applications
      • Flow Cytometry
      Biological Information
      CloneMB1.2
      HostRat
      SpecificityAntibody recognizes integrin β1.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
      Gene Symbol
      • ITGB1
      • GPIIA
      • OTTHUMP00000063731
      • OTTHUMP00000046253
      • OTTHUMP00000063733
      • VLAB
      • OTTHUMP00000063732
      • OTTHUMP00000046253
      • MDF2
      • FNRB
      • CD29
      • MSK12
      • VLA-BETA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation.

      SUBUNIT STUCTURE: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5. Interacts with FLNA, FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with KRT1 in the presence of GNB2L1 and SRC. Interacts with HIV-1 Tat. Binds to human echoviruses 1 and 8 capsid proteins and acts as a receptor for these viruses.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note= Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells.

      TISSUE SPECIFICTY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).

      PTM: The cysteine residues are involved in intrachain disulfide bonds By similarity.

      SIMILARITY: Belongs to the integrin beta chain family.
      Molecular Weight88 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using NIH/3T3
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB269F 04053252681356

      Documentation

      Milli-Mark™ Anti-Integrin β1 (CD29)-FITC conjugate Antibody, clone MB1.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Milli-Mark™ Anti-Integrin β1 (CD29)-FITC conjugate Antibody, clone MB1.2 Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti Mouse Integrin #946;1 (CD29)-FITC, clone MB1.2 - 2121805 2121805
      Milli-Mark Anti Mouse Integrin #946;1 (CD29)-FITC, clone MB1.2 - NRG1830298 NRG1830298
      Milli-Mark Anti Mouse Integrin 1 (CD29)-FITC, clone MB1.2 - 2024546 2024546
      Milli-Mark Anti Mouse Integrin 1 (CD29)-FITC, clone MB1.2 - 2266755 2266755
      Milli-Mark Anti Mouse Integrin 1 (CD29)-FITC, clone MB1.2 - NG1906022 NG1906022
      Milli-Mark Anti Mouse Integrin 1 (CD29)-FITC, clone MB1.2 - NG1914819 NG1914819
      Milli-Mark Anti Mouse Integrin 1 (CD29)-FITC, clone MB1.2 - NG1940539 NG1940539
      Milli-Mark™ Anti Mouse Integrin β1 (CD29)-FITC, clone MB1.2 - 3365624 3365624
      Milli-Mark™ Anti Mouse Integrin β1 (CD29)-FITC, clone MB1.2 - 3610576 3610576
      Milli-Mark™ Anti Mouse Integrin β1 (CD29)-FITC, clone MB1.2 - 4087892 4087892
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      References

      Reference overviewPub Med ID
      Spatiotemporal control of phosphatidylinositol 4-phosphate by Sac2 regulates endocytic recycling.
      Hsu, F; Hu, F; Mao, Y
      The Journal of cell biology  209  97-110  2015

      Show Abstract       		25869669 25869669