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475954 MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem

The MRN-ATM Pathway Inhibitor, Mirin, also referenced under CAS 299953-00-7, controls the biological activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex. This small molecule/inhibitor is primarily used for Cell Structure applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin

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475954
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
299953-00-7C₁₀H₈N₂O₂S

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475954-10MG
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      Description
      OverviewA cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
      Catalogue Number475954
      Brand Family Calbiochem®
      Synonyms(Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
      References
      ReferencesGarner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
      Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
      Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.
      Product Information
      CAS number299953-00-7
      FormReddish orange powder
      Hill FormulaC₁₀H₈N₂O₂S
      Chemical formulaC₁₀H₈N₂O₂S
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      475954-10MG 04055977184297

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem Certificates of Analysis

      TitleLot Number
      475954

      References

      Reference overview
      Garner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
      Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
      Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-August-2009 RFH
      Synonyms(Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
      DescriptionA cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
      FormReddish orange powder
      Intert gas (Yes/No) Packaged under inert gas
      CAS number299953-00-7
      Chemical formulaC₁₀H₈N₂O₂S
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (10 mg/ml) or Ethanol (3 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesGarner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
      Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
      Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.