Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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444217
Sigma-AldrichMMP-3, Catalytic Domain, Human, Recombinant, E. coli
Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952. Wilhelm, S.M., et al. 1993. J. Biol. Chem. 268, 21906. Ye, Q.Z., et al. 1992. Biochemistry 31, 11231.
Product Information
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH₂ per min at 37°C, pH 7.0.
EC number
3.4.24.17
Form
Liquid
Formulation
In 50 mM Tris-HCl, 10 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 7.5.
MMP-3, Catalytic Domain, Human, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
444217
References
Reference overview
Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952. Wilhelm, S.M., et al. 1993. J. Biol. Chem. 268, 21906. Ye, Q.Z., et al. 1992. Biochemistry 31, 11231.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Recombinant, human MMP-3 catalytic domain expressed in E. coli.
Form
Liquid
Formulation
In 50 mM Tris-HCl, 10 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 7.5.
EC number
3.4.24.17
Purity
≥90% by SDS-PAGE
Contaminants
no other MMP activity detected
Specific activity
≥1250 mU/mg protein
Unit definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH₂ per min at 37°C, pH 7.0.
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952. Wilhelm, S.M., et al. 1993. J. Biol. Chem. 268, 21906. Ye, Q.Z., et al. 1992. Biochemistry 31, 11231.
