Millipore Sigma Vibrant Logo

RMHE-120K MILLIPLEX® Rat Metabolic Hormone Expanded Panel - Metabolism Multiplex Assay

The MILLIPLEX® Rat Metabolic Hormone Expanded Panel is a bead-based quantitative assay based on the Luminex® xMAP® technology, which enables the simultaneous analysis of up to 14 analytes in a 25 µL s

 More>>
View Products on Sigmaaldrich.com
RMHE-120K
Design And Price Your Kit

Overview

Description
Catalogue NumberRMHE-120K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Rat Metabolic Hormone Expanded Panel - Metabolism Multiplex Assay
OverviewMILLIPLEX® Rat Metabolic Hormone Expanded Panel is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/lysate and culture supernatant samples and rat serum or plasma: Active Amylin, C-peptide 2, Active Ghrelin, Gastric Inhibitory Polypeptide (GIP), Glucagon-like peptide-1 (GLP-1) Active or Total, Glucagon, Interleukin-6 (IL-6), Insulin, Leptin, Monocyte Chemoattractant Protein-1 (MCP-1), Pancreatic Polypeptide (PP), Peptide YY (PYY), Secretin, and Tumor Necrosis Factor alpha (TNFα). Notes: GLP-1 (Active) and GLP-1 (Total) cannot be run together in the same assay. Protease inhibitors should be added upon collection of samples.
Background InformationMILLIPLEX® Rat Metabolic Hormone Expanded Panel is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/lysate and culture supernatant samples and rat serum or plasma: Active Amylin, C-peptide 2, Active Ghrelin, Gastric Inhibitory Polypeptide (GIP), Glucagon-like peptide-1 (GLP-1) Active or Total, Glucagon, Interleukin-6 (IL-6), Insulin, Leptin, Monocyte Chemoattractant Protein-1 (MCP-1), Pancreatic Polypeptide (PP), Peptide YY (PYY), Secretin, and Tumor Necrosis Factor alpha (TNFα). Notes: GLP-1 (Active) and GLP-1 (Total) cannot be run together in the same assay. Protease inhibitors should be added upon collection of samples.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <10%
  • Inter-assay: <20%
Multiplex Ordering RequirementsGLP-1 (Active) and GLP-1 (Total) cannot be run together in the same assay.
Panel TypeMAGNETIC Metabolism
Quality LevelMQ200
Applications
ApplicationThe MILLIPLEX® Rat Metabolic Hormone Expanded Panel is a bead-based quantitative assay based on the Luminex® xMAP® technology, which enables the simultaneous analysis of up to 14 analytes in a 25 µL sample volume.
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay requiring 25 µL rat serum, plasma, or cell/tissue culture supernatants or lysates. • No sample dilution is required.
Biological Information
Species Reactivity
  • Rat
Analytes Available
  • Amylin (Active)
  • C-Peptide 2
  • Ghrelin (Active)
  • GIP
  • GLP-1 (Active)
  • GLP-1 (Total)
  • Glucagon
  • IL-6
  • Insulin
  • Leptin
  • MCP-1/CCL2
  • Pancreatic Polypeptide
  • PYY (total)
  • Secretin
  • TNF-α
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies
and any of the other analytes in this panel.
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of each analyte.
Standard Curve Range
  • Amylin (active): 41-30,000 pg/mL
  • C-Peptide 2: 69-50,000 pg/mL
  • Ghrelin (active): 4.1-3,000 pg/mL
  • GIP (total): 4.1-3,000 pg/mL
  • GLP-1 (active): 27-20,000 pg/mL
  • GLP-1 (total): 27-20,000 pg/mL
  • Glucagon: 2.7-2,000 pg/mL
  • IL-6: 110-80,000 pg/mL
  • Insulin: 110-80,000 pg/mL
  • Leptin: 48-35,000 pg/mL
  • MCP-1/CCL2: 69-50,000 pg/mL
  • Pancreatic Polypeptide (PP): 6.9-5,000 pg/mL
  • PYY (total): 6.9-5,000 pg/mL
  • Secretin: 6.9-5,000 pg/mL
  • TNFα: 1.4-1,000 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Global Trade Item Number
Catalogue Number GTIN
RMHE-120K 04065268968307