Millipore Sigma Vibrant Logo

HSP2MAG-63K MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 2 - Immune Response Multiplex Assay

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology
View Products on Sigmaaldrich.com
HSP2MAG-63K
Design And Price Your Kit

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Granzyme B HSP70 IL-1α IL-8 MIP-1α MIP-1β MMP-8 HMplexLuminex xMAP
Description
Catalogue NumberHSP2MAG-63K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Sepsis Magnetic Bead Panel 2 - Immune Response Multiplex Assay
Background InformationSepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay: < 10
Panel TypeMAGNETIC Immune Response
Quality LevelMQ200
Applications
ApplicationInflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is a 2-hour incubation assay.
• This assay requires 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Granzyme B
  • HSP70
  • IL-1α
  • IL-8
  • MIP-1α
  • MIP-1β
  • MMP-8
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • Granzyme B 95%
  • HSP70 92%
  • IL-1α 97%
  • IL-8 98%
  • MIP-1α 92%
  • MIP-1β 87%
  • MMP-8 92%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for individual sensitivities.
Standard Curve Range
  • Granzyme B: 2 - 2,000 pg/mL
  • HSP70: 2,930 - 3,000,000 pg/mL
  • IL-1α and MIP-1β: 10 - 10,000 pg/mL
  • IL-8 and MIP-1β: 5 - 5,000 pg/mL
  • MMP-8: 195 - 200,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HSP2MAG-63K 04053252987649