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SCC064SF LX-2 Hepatic Stellate Cell Line-SF Adapted

Serum-Free media adapted LX-2 human hepatic stellate cells that been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making them a highly suitable model of human hepatic fibrosis.

NewSCC064SF
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC064SF
      DescriptionLX-2 Hepatic Stellate Cell Line-SF Adapted
      Alternate Names
      • HSC
      • Perisinusoidal cells
      • Ito cells
      • Hepatic lipocytes
      • Hepatic pericytes
      Background InformationHepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

      LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

      Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

      The LX-2 Hepatic Stellate Cell Line-SF Adapted cell ine (SCC064SF) has been preadapted to serum-free media conditions using the LX-2 Human Hepatic Stellate Serum-Free Media (SCM064SFM). It is recommended to use SCM064SFM to thaw and expand the cell line.
      References
      Product Information
      Components
      • 1) ≥1X106 viable LX-2 Human Hepatic Stellate Cells-SF Adapted: (Catalog No. SCC064SF). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationSerum-Free media adapted LX-2 human hepatic stellate cells that been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making them a highly suitable model of human hepatic fibrosis.
      Key Applications
      • Stem Cell Culture
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      HostHuman
      Cell Line Type
      • Hepatocyte Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases tested by using the Essential CLEAR Panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of human origin and negative for interspecies contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsLX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC064SF 04065272306249