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389561 Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

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389561
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389561-100U
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      Description
      OverviewNative hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.
      Catalogue Number389561
      Brand Family Calbiochem®
      References
      ReferencesBaker, J.R., et al. 2002. Biochem. J. 365, 317.
      Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
      Product Information
      CAS number37326-33-3
      Activity≥ 100 U/ampule [1 U = 1 TRU (Turbidity Reducing Unit)]
      Unit of DefinitionOne TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
      EC number3.2.1.35
      FormWhite lyophilized powder
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥2000 U/A280
      Physicochemical Information
      ContaminantsProtease: ≤ 0.05 units/ampule (pH 6.0)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      389561-100U 07790788058128

      Documentation

      Hyaluronidase, Streptomyces hyaluronlyticus nov. sp. SDS

      Title

      Safety Data Sheet (SDS) 

      Hyaluronidase, Streptomyces hyaluronlyticus nov. sp. Certificates of Analysis

      TitleLot Number
      389561

      References

      Reference overview
      Baker, J.R., et al. 2002. Biochem. J. 365, 317.
      Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-July-2009 JSW
      DescriptionNative hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.

      Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.
      FormWhite lyophilized powder
      Recommended reaction conditionsAssay for Enzymatic Activity Reagent Preparation • Prepare acetate buffer: 150 mM NaCl, 20 mM sodium acetate-acetic acid buffer, pH 6.0. • Prepare hyaluronic acid solution: 50 mg purified potassium hyaluronate (containing approximately 38% glucuronic acid) is dissolved in 100 ml of the above mentioned sodium acetate buffer. • Measure acidic bovine serum albumin with a spectrophotometer at 280 nm. Adjust the total absorbance of the albumin solution to 910. Dilute to 100 ml with 0.5 N acetate buffer, pH 4.3. Adjust the pH of the albumin solution to 3.1 using 4 N HCl. Boil in a water bath for 30 min at 100°C. Adjust to 100 ml with distilled H2O. This solution is stable for 3-4 weeks at 4°C. To use this solution, dilute with 5 volumes of acetate buffer from above. Activity Protocol • For the assay, mix 0.5 ml of the enzyme solution (made with acetate buffer without NaCl) with 0.5 ml of the hyaluronic acid solution. Incubate for 30 min at 60°C. • Add 4 ml of the acidic bovine serum albumin solution. Incubate for 10 min at 60°C. • Measure the turbidity at 660 nm. Obtain a blank value by using deactivated enzyme (incubate at 100°C for 10 min). Correct the reading by subtracting the blank value. (blank value - measured value/blank value) x 2 x 2 x dilution = TRU/ml
      CAS number37326-33-3
      EC number3.2.1.35
      ContaminantsProtease: ≤ 0.05 units/ampule (pH 6.0)
      Specific activity≥2000 U/A280
      Activity≥ 100 U/ampule [1 U = 1 TRU (Turbidity Reducing Unit)]
      Unit definitionOne TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
      Solubility500 mM Acetate buffer, pH 6.0 (30 U/ml) or H₂O (30 U/ml)
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
      Toxicity Standard Handling
      Merck USA index14, 4758
      ReferencesBaker, J.R., et al. 2002. Biochem. J. 365, 317.
      Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.